Summary.
Flow cytometric analysis of mitochondria isolated from maize leaves revealed two distinct rhodamine-123-stained fluorescence populations distinguishable by their main fluorescence channel. Further microscopic observation of mitochondria stained with Janus Green B and rhodamine-123 revealed the occurrence of differently sized mitochondrial particles. It was shown by pulsed-field gel electrophoresis that the DNA from the isolated mitochondria ranged in size from 45 to 100 kb. These results suggest that different types of mitochondria with different physiological status, mass, and genomic DNA size probably coexist and carry out different physiological functions throughout the whole process of maize leaf growth and development.
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References
A Almeida C Lopez-Mediavilla A Orfao JM Medina (1994) ArticleTitlePostnatal changes in rhodamine-123 stained mitochondrial populations are sensitive to protein synthesis inhibitors but mimicked in vitro by ATP FEBS Lett 344 50–54 Occurrence Handle8181564 Occurrence Handle10.1016/0014-5793(94)00345-9 Occurrence Handle1:CAS:528:DyaK2cXktVGrtLg%3D
CP Andre V Walbot (1995) ArticleTitlePulsed-field gel mapping of maize mitochondrial chromosomes Mol Gen Genet 247 255–263 Occurrence Handle7753036 Occurrence Handle10.1007/BF00705657 Occurrence Handle1:CAS:528:DyaK2MXlvVWmtbs%3D
A Baracca G Sgarbi G Solaini G Lenaz (2003) ArticleTitleRhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F (0) during ATP synthesis Biochim Biophys Acta 1606 137–146 Occurrence Handle14507434 Occurrence Handle10.1016/S0005-2728(03)00110-5 Occurrence Handle1:CAS:528:DC%2BD3sXnsVagurs%3D
S Braun M Erdelyi A Udvardy (1967) ArticleTitleJanus green B and the biologic “oxygen effect” Cancer Res 27 660–667 Occurrence Handle6025729 Occurrence Handle1:CAS:528:DyaF2sXktFSisL4%3D
DR Green G Kroemer (2004) ArticleTitleThe pathophysiology of mitochondrial cell death Science 305 626–629 Occurrence Handle15286356 Occurrence Handle10.1126/science.1099320 Occurrence Handle1:CAS:528:DC%2BD2cXmtFWqsrc%3D
MR Hanson S Bentolila (2004) ArticleTitleInteractions of mitochondrial and nuclear genes that affect male gametophyte development Plant Cell 16 S154–S169 Occurrence Handle15131248 Occurrence Handle10.1105/tpc.015966 Occurrence Handle1:CAS:528:DC%2BD2cXlsFWltb4%3D
R Hernandez-Berciano MV Garcia C Lopez-Mediavilla A Orfao JM Medina (1993) ArticleTitleChanges in mitochondrial rhodamine-123-fluorescence populations of rat hepatocytes in primary culture Exp Cell Res 209 82–88 Occurrence Handle8224011 Occurrence Handle10.1006/excr.1993.1288 Occurrence Handle1:STN:280:ByuD3sblslA%3D
F Hochholdinger L Guo PS Schnable (2004) ArticleTitleCytoplasmic regulation of the accumulation of nuclear-encoded proteins in the mitochondrial proteome of maize Plant J 37 199–208 Occurrence Handle14690504 Occurrence Handle1:CAS:528:DC%2BD2cXhsVGltbg%3D
PK Kennady MG Ormerod S Singh G Pande (2004) ArticleTitleVariation of mitochondrial size during the cell cycle: a multiparameter flow cytometric and microscopic study Cytometry A 62 97–108 Occurrence Handle15536637 Occurrence Handle10.1002/cyto.a.20091
SS Li (1997) ArticleTitleBreeding of maize inbred line Huangzao 4 and it applications Beijing Agric Sci 15 19–21
C Lopez-Mediavilla A Orfao M Gonzalez JM Medina (1989) ArticleTitleIdentification by flow cytometry of two distinct rhodamine-123-stained mitochondrial populations in rat liver FEBS Lett 254 115–120 Occurrence Handle2476332 Occurrence Handle10.1016/0014-5793(89)81020-8 Occurrence Handle1:CAS:528:DyaL1MXlslWksro%3D
C Lopez-Mediavilla A Orfao J San Miguel JM Medina (1992) ArticleTitleDevelopmental changes in rat liver mitochondrial populations analyzed by flow cytometry Exp Cell Res 203 134–140 Occurrence Handle1426036 Occurrence Handle10.1016/0014-4827(92)90048-D Occurrence Handle1:STN:280:ByyD2cbovF0%3D
C Lopez-Mediavilla A Orfao MV Garcia JM Medina (1995) ArticleTitleChanges in adult rat liver mitochondrial populations at different energy states analyzed by flow cytometry Biochim Biophys Acta 1232 27–32 Occurrence Handle7495835 Occurrence Handle10.1016/0005-2728(95)00105-8
G Mattiasson (2004) ArticleTitleAnalysis of mitochondrial generation and release of reactive oxygen species Cytometry A 62 89–96 Occurrence Handle15517564 Occurrence Handle10.1002/cyto.a.20089
JM Medina C Lopez-Mediavilla A Orfao (2002) ArticleTitleFlow cytometry of isolated mitochondria during development and under some pathological conditions FEBS Lett 510 127–132 Occurrence Handle11801239 Occurrence Handle10.1016/S0014-5793(01)03260-4 Occurrence Handle1:CAS:528:DC%2BD38XktFeitQ%3D%3D
DD Newmeyer S Ferguson-Miller (2003) ArticleTitleMitochondria: releasing power for life and unleashing the machineries of death Cell 112 481–490 Occurrence Handle12600312 Occurrence Handle10.1016/S0092-8674(03)00116-8 Occurrence Handle1:CAS:528:DC%2BD3sXhs1SnsLs%3D
P Petit P Diolez P Muller SC Brown (1986) ArticleTitleBinding of concanavalin A to the outer membrane of potato tuber mitochondria detected by flow cytometry FEBS Lett 196 65–70 Occurrence Handle10.1016/0014-5793(86)80215-0 Occurrence Handle1:CAS:528:DyaL28XhtVCkt7w%3D
DP Sirk Z Zhu JS Wadia LR Mills (2003) ArticleTitleFlow cytometry and GFP: a novel assay for measuring the import and turnover of nuclear-encoded mitochondrial proteins in live PC12 cells Cytometry A 56 15–22 Occurrence Handle14566935 Occurrence Handle10.1002/cyto.a.10084
B Yang N Keshelava CP Anderson CP Reynolds (2003) ArticleTitleAntagonism of buthionine sulfoximine cytotoxicity for human neuroblastoma cell lines by hypoxia is reversed by the bioreductive agent tirapazamine Cancer Res 63 1520–1526 Occurrence Handle12670899 Occurrence Handle1:CAS:528:DC%2BD3sXisFaitrc%3D
N Yao BJ Eisfelder J Marvin JT Greenberg (2004) ArticleTitleThe mitochondrion – an organelle commonly involved in programmed cell death in Arabidopsis thaliana Plant J 40 596–610 Occurrence Handle15500474 Occurrence Handle10.1111/j.1365-313X.2004.02239.x Occurrence Handle1:CAS:528:DC%2BD2cXhtVyit7jP
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Correspondence: Lijia Li, Key Laboratory for Plant Developmental Biology of the Ministry of Education, College of Life Sciences, Wuhan University, Wuhan 430072, People’s Republic of China.
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Yang, J., Ma, L., Zhang, Y. et al. Flow cytometric identification of two different rhodamine-123-stained mitochondrial populations in maize leaves. Protoplasma 231, 249–252 (2007). https://doi.org/10.1007/s00709-007-0259-6
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DOI: https://doi.org/10.1007/s00709-007-0259-6