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In situ localization of three cDNA sequences associated with the later stages of aposporic embryo sac development of Brachiaria brizantha

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Summary.

Brachiaria brizantha is a forage grass of African origin, highly cultivated in the Brazilian tropics for beef cattle production. We have analyzed the temporal and spatial expression of cDNA sequences by in situ hybridization in ovaries of apomictic and sexual plants. The studied sequences share molecular identity with myosin, aquaporin, and mitogen-activated protein kinase and were named BbrizMYO, BbrizAQP, and BbrizMAPK, respectively. BbrizMYO was expressed in apomictic and sexual embryo sacs, but somewhat later in the Polygonum type embryo sacs of sexual plants. BbrizAQP and BbrizMAPK transcripts were restricted to the Panicum type embryo sacs of apomictic plants; BbrizMAPK, in synergids; and BbrizAQP, also in different ovular cells during development. The common feature that arose from the analysis of the expression patterns of these three sequences was significant expression in the synergids. Their putative role in the maturation of Panicum type embryo sacs of apomictic plants and embryo development is discussed in view of the characteristics of apomictic reproduction.

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References

  • E Albertini G Marconi G Barcaccia L Raggi M Falcinelli (2004) ArticleTitleIsolation of candidate genes for apomixis in Poa pratensis L Plant Mol Biol 56 879–894 Occurrence Handle15821987 Occurrence Handle10.1007/s11103-004-5211-y Occurrence Handle1:CAS:528:DC%2BD2MXjtV2gsL4%3D

    Article  PubMed  CAS  Google Scholar 

  • E Albertini G Marconi L Reale G Barcaccia A Porceddu F Ferranti M Falcinelli (2005) ArticleTitleSERK and APOSTART. Candidate genes for apomixis in Poa pratensis Plant Physiol 138 2185–2199 Occurrence Handle16024690 Occurrence Handle10.1104/pp.105.062059 Occurrence Handle1:CAS:528:DC%2BD2MXps12lsb4%3D

    Article  PubMed  CAS  Google Scholar 

  • B Alberts D Bray J Lewis M Raff K Roberts JD Watson (1994) Biologia molecular da célula EditionNumber3 Artes Médicas Porto Alegre

    Google Scholar 

  • ER Alves (2000) Aspectos da reprodução em Brachiaria brizantha cv. Marandu Mestrado em Botânica, Universidade de Brasília Brasília, Brazil

    Google Scholar 

  • ER Alves VTC Carneiro ACG Araujo (2001) ArticleTitleDirect evidence of pseudogamy in an apomictic Brachiaria brizantha (Poaceae) Sex Plant Reprod 14 207–212 Occurrence Handle10.1007/s00497-001-0120-6

    Article  Google Scholar 

  • ACG Araujo S Mukhambetzhanov MT Pozzobon EF Santana VTC Carneiro (2000) ArticleTitleFemale gametophyte development in apomictic and sexual Brachiaria brizantha (Poaceae) Rev Cytol Biol Veg 23 13–28

    Google Scholar 

  • SE Asker L Jerling (1992) Apomixis in plants CRC Press Boca Raton, Fla

    Google Scholar 

  • F Barrieu F Chaumont MJ Chrispeels (1998) ArticleTitleHigh expression of the tonoplast aquaporin ZmTIP1 in epidermal and conducting tissues of maize 1 Plant Physiol 117 1153–1163 Occurrence Handle9701571 Occurrence Handle10.1104/pp.117.4.1153 Occurrence Handle1:CAS:528:DyaK1cXlsFaqsb0%3D

    Article  PubMed  CAS  Google Scholar 

  • PA Burris Y Zhang JC Rusconi V Corbin (1998) ArticleTitleThe pore-forming and cytoplasmic domains of the neurogenic gene product, BIG BRAIN, are conserved between Drosophila virilis and Drosophila melanogaster Gene 206 69–76 Occurrence Handle9461417 Occurrence Handle10.1016/S0378-1119(97)00567-2 Occurrence Handle1:CAS:528:DyaK1cXlslWksg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • JG Carman (1997) ArticleTitleAsynchronous expression of duplicate genes in angiosperms may cause apomixis, bispory, tetraspory, and polyembryony Biol J Linn Soc 61 51–94 Occurrence Handle10.1006/bijl.1996.0118

    Article  Google Scholar 

  • F Chaumont F Barrieu E Wojcik MJ Chrispeels R Jung (2001) ArticleTitleAquaporins constitute a large and highly divergent protein family in maize Plant Physiol 125 1206–1215 Occurrence Handle11244102 Occurrence Handle10.1104/pp.125.3.1206 Occurrence Handle1:CAS:528:DC%2BD3MXitFWrt7o%3D

    Article  PubMed  CAS  Google Scholar 

  • L Chen C Miyazaki A Kojima A Saito T Adachi (1999) ArticleTitleIsolation and characterization of a gene expressed during early embryo sac development in apomictic guinea grass (Panicum maximum) J Plant Physiol 154 55–62 Occurrence Handle1:CAS:528:DyaK1MXhtVOhuro%3D

    CAS  Google Scholar 

  • L Chen L Guan M Seo F Hoffmann T Adachi (2005) ArticleTitleDevelopmental expression of ASG-1 during gametogenesis in apomictic guinea grass (Panicum maximum) J Plant Physiol 162 1141–1148 Occurrence Handle16255172 Occurrence Handle10.1016/j.jplph.2005.02.010 Occurrence Handle1:CAS:528:DC%2BD2MXht1yktrrO

    Article  PubMed  CAS  Google Scholar 

  • MJ Chrispeels C Maurel (1994) ArticleTitleAquaporins: the molecular basis of facilitated water movement through living plant cells? Plant Physiol 105 9–13 Occurrence Handle7518091 Occurrence Handle10.1104/pp.105.1.9 Occurrence Handle1:CAS:528:DyaK2cXjtF2gsb0%3D

    Article  PubMed  CAS  Google Scholar 

  • MJ Chrispeels NM Crawford JI Schroeder (1999) ArticleTitleProteins for transport of water and mineral nutrients across the membranes of plant cells Plant Cell 11 661–675 Occurrence Handle10213785 Occurrence Handle10.1105/tpc.11.4.661 Occurrence Handle1:CAS:528:DyaK1MXjtFSrurs%3D

    Article  PubMed  CAS  Google Scholar 

  • VT Ciavatta R Morillon GS Pullman MJ Chrispeels J Cairney (2001) ArticleTitleAn aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine Plant Physiol 127 1556–1567 Occurrence Handle11743100 Occurrence Handle10.1104/pp.127.4.1556 Occurrence Handle1:CAS:528:DC%2BD38XjtVWnsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • A De Lozanne JA Spudich (1987) ArticleTitleDisruption of the Dictyostelium myosin heavy chain gene by homologous recombination Science 236 1086–1091 Occurrence Handle3576222 Occurrence Handle10.1126/science.3576222 Occurrence Handle1:CAS:528:DyaL2sXktlCht7k%3D

    Article  PubMed  CAS  Google Scholar 

  • V Decroocq-Ferrant J van Went MW Bianchi SC De Vries M Kreis (1995) ArticleTitlePetunia hybrida homologues of shaggy/zeste-white 3 expressed in female and male reproductive organs Plant J 7 897–911 Occurrence Handle7599650 Occurrence Handle10.1046/j.1365-313X.1995.07060897.x Occurrence Handle1:CAS:528:DyaK2MXnsF2ltrg%3D

    Article  PubMed  CAS  Google Scholar 

  • do Valle CB (1986) Cytology, mode of reproduction and forage quality of selected species of Brachiaria Griseb. PhD thesis, University of Illinois, Urbana, Ill

  • CB do Valle Y Savidan (1996) Genetics, cytogenetics and reproductive biology of Brachiaria JW Miles BL Maass CB do Valle (Eds) Brachiaria: biology, agronomy and improvement Centro Internacional de Agrocultura Tropical and Empresa Brasileira de Pesquisa Agropecuária Cali, Colombia and Campo Grande, Brazil 147–163

    Google Scholar 

  • Dusi DMA (2001) Apomixis in Brachiaria decumbens Stapf. PhD thesis, University of Wageningen, Wageningen, the Netherlands

  • DMA Dusi MTM Willemse (1999a) ArticleTitleActivity and localisation of sucrose synthase and invertase in ovules of sexual and apomictic Brachiaria decumbens Protoplasma 208 173–185 Occurrence Handle10.1007/BF01279088 Occurrence Handle1:CAS:528:DC%2BD3cXoslSmtQ%3D%3D

    Article  CAS  Google Scholar 

  • DMA Dusi MTM Willemse (1999b) ArticleTitleApomixis in Brachiaria decumbens Stapf.: gametophytic development and reproductive calendar Acta Biol Cracov Ser Bot 41 151–162

    Google Scholar 

  • MJ Fisher PC Kerridge (1996) The agronomy and physiology of Brachiaria species JW Miles BL Maass CB do Valle (Eds) Brachiaria: biology, agronomy and improvement Centro Internacional de Agricultura Tropical and Empresa Brasileira de Pesquisa Agropecuária Cali, Colombia and Campo Grande, Brazil 43–52

    Google Scholar 

  • WE Friedman (2006) ArticleTitleEmbryological evidence for developmental lability during early angiosperm evolution Nature 441 337–340 Occurrence Handle16710419 Occurrence Handle10.1038/nature04690 Occurrence Handle1:CAS:528:DC%2BD28XksleltLw%3D

    Article  PubMed  CAS  Google Scholar 

  • Y Fu M Yuan BQ Huang HY Yang SY Zee TP O’Brien (2000) ArticleTitleChanges in actin organization in the living egg apparatus of Torenia fournieri during fertilization Sex Plant Reprod 12 315–322 Occurrence Handle10.1007/s004970000026 Occurrence Handle1:CAS:528:DC%2BD3cXmtlSkt7o%3D

    Article  CAS  Google Scholar 

  • D Grimanelli O Leblanc E Perotti U Grossniklaus (2001) ArticleTitleDevelopmental genetics of gametophytic apomixis Trends Genet 17 597–604 Occurrence Handle11585667 Occurrence Handle10.1016/S0168-9525(01)02454-4 Occurrence Handle1:CAS:528:DC%2BD3MXnt1Wju7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • I Hakman P Oliviusson (2002) ArticleTitleHigh expression of putative aquaporin genes in cells with transporting and nutritive functions during seed development in Norway spruce (Picea abies) J Exp Bot 53 639–649 Occurrence Handle11886883 Occurrence Handle10.1093/jexbot/53.369.639 Occurrence Handle1:CAS:528:DC%2BD38XitlCks74%3D

    Article  PubMed  CAS  Google Scholar 

  • WW Hanna EC Bashaw (1987) ArticleTitleApomixis: its identification and use in plant breeding Crop Sci 27 1136–1139 Occurrence Handle10.2135/cropsci1987.0011183X002700060010x

    Article  Google Scholar 

  • E Heberle-Bors (2001) ArticleTitleCyclin-dependent protein kinases, mitogen-activated protein kinases and the plant cell cycle Curr Sci 80 225–232 Occurrence Handle1:CAS:528:DC%2BD3MXhsVWrs7k%3D

    CAS  Google Scholar 

  • I Herskowitz (1995) ArticleTitleMAP kinase pathways in yeast: for mating and more Cell 80 187–197 Occurrence Handle7834739 Occurrence Handle10.1016/0092-8674(95)90402-6 Occurrence Handle1:CAS:528:DyaK2MXjtlGnsrw%3D

    Article  PubMed  CAS  Google Scholar 

  • T Higashiyama (2002) ArticleTitleThe synergid cell: attractor and acceptor of the pollen tube for double fertilization J Plant Res 115 149–160 Occurrence Handle12884138 Occurrence Handle10.1007/s102650200020

    Article  PubMed  Google Scholar 

  • WA Jensen DB Fisher (1968) ArticleTitleCotton embryogenesis: entrance and discharge of the pollen tube in the embryo sac Planta 78 158–183 Occurrence Handle10.1007/BF00406648

    Article  Google Scholar 

  • C Jonak A Páy L Bögre H Hirt E Heberle-Bors (1993) ArticleTitleThe plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner Plant J 3 611–617 Occurrence Handle8220466 Occurrence Handle10.1046/j.1365-313X.1993.03040611.x Occurrence Handle1:CAS:528:DyaK3sXmt1Wlsbw%3D

    Article  PubMed  CAS  Google Scholar 

  • Kapil RN, Bhatnagar AK (1975) A fresh look at the process of double fertilization in angiosperms. Phytomorphology Silver Jubilee: 334–368

  • AM Koltunow U Grossniklaus (2003) ArticleTitleApomixis: a developmental perspective Annu Rev Plant Biol 54 547–574 Occurrence Handle14503003 Occurrence Handle10.1146/annurev.arplant.54.110901.160842 Occurrence Handle1:CAS:528:DC%2BD3sXntFSnsrk%3D

    Article  PubMed  CAS  Google Scholar 

  • M Koscinska-Pajak J Bednara (2006) ArticleTitleUnusual microtubular cytoskeleton of apomictic embryo sac of Chondrilla juncea L Protoplasma 227 87–93 Occurrence Handle16736250 Occurrence Handle10.1007/s00709-006-0147-5 Occurrence Handle1:STN:280:DC%2BD28zgvVCnuw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • O Leblanc I Armstead S Pessino JPA Ortiz C Evans CB do Valle MD Hayward (1997) ArticleTitleNon-radioactive mRNA fingerprinting to visualize gene expression in mature ovaries of Brachiaria hybrids derived from B. brizantha, an apomictic tropical forage Plant Sci 126 49–58 Occurrence Handle10.1016/S0168-9452(97)00067-8 Occurrence Handle1:CAS:528:DyaK2sXkslWjtLY%3D

    Article  CAS  Google Scholar 

  • H Lodish A Berk SL Zipursky P Matsudaira D Baltimore JE Darnell (2001) Molecular cell biology EditionNumber4 WH Freeman and Company New York

    Google Scholar 

  • S Lutts J Ndikumana BP Louant (1994) ArticleTitleMale and female sporogenesis and gametogenesis in apomictic Brachiaria brizantha, Brachiaria decumbens and F1 hybrids with sexual colchicine induced tetraploid Brachiaria ruziziensis Euphytica 78 19–25

    Google Scholar 

  • JW Miles BL Maass CB do Valle (Eds) (1996) Brachiaria: biology, agronomy and improvement Centro Internacional de Agricultura Tropical and Empresa Brasileira de Pesquisa Agropecuária Cali, Colombia and Campo Grande, Brazil

    Google Scholar 

  • HL Mogensen (1978) ArticleTitlePollen tube-synergid interactions in Proboscidea louisianica (Martineaceae) Am J Bot 65 953–964 Occurrence Handle10.2307/2442682

    Article  Google Scholar 

  • Ngendahayo M (1988) Méchanismes de la reproduction dans le genre Brachiaria. PhD thesis, Université Catholique de Louvain, Louvain, Belgium

  • GA Nogler (1984) Gametophytic apomixis BM Johri (Eds) Embryology of angiosperms Springer Berlin Heidelberg New York 475–518

    Google Scholar 

  • GA Nogler (1994) ArticleTitleGenetics of gametophytic apomixis – a historical sketch Pol Bot Stud 8 5–11

    Google Scholar 

  • Penteado MIO, Santos ACM, Rodrigues IF, do Valle CB, Seixas MAC, Esteves A (2000) Determinação de ploidia e avaliação da quantidade de DNA total em diferentes espécies do gênero Brachiaria. Embrapa Gado de Corte, Boletim de Pesquisa, vol 11. Empresa Brasileira de Pesquisa Agropecuária Gado de Corte, Campo Grande, Brazil

  • SC Pessino F Espinoza EJ Martinez JP Ortiz EM Valle CL Quarin (2001) ArticleTitleIsolation of cDNA clones differentially expressed in flowers of apomictic and sexual Paspalum notatum Hereditas 134 35–42 Occurrence Handle11525063 Occurrence Handle10.1111/j.1601-5223.2001.00035.x Occurrence Handle1:CAS:528:DC%2BD3MXmvV2rs7w%3D

    Article  PubMed  CAS  Google Scholar 

  • JCM Rodrigues GB Cabral DMA Dusi LV Mello D Rigden VTC Carneiro (2003) ArticleTitleIdentification of differentially expressed cDNA sequences in ovaries of sexual and apomictic plants of Brachiaria brizantha Plant Mol Biol 53 745–757 Occurrence Handle15082923 Occurrence Handle10.1023/B:PLAN.0000023664.21910.bd Occurrence Handle1:CAS:528:DC%2BD2cXjtVWmsbo%3D

    Article  PubMed  CAS  Google Scholar 

  • SD Russell (1992) Double fertilization SD Russell C Dumas (Eds) Sexual reproduction in flowering plants SeriesTitleInternational review of cytology NumberInSeries140 Academic Press London 357–388

    Google Scholar 

  • SD Russell (1993) ArticleTitleThe egg cell: development and role in fertilization and early embryogenesis Plant Cell 5 1349–1359 Occurrence Handle12271034 Occurrence Handle10.1105/tpc.5.10.1349

    Article  PubMed  Google Scholar 

  • Serrão EAD, Simão Neto M (1971) Informações sobre duas espécies de gramíneas forrageiras do gênero Brachiaria na Amazônia: B. decumbens Stapf e B. ruziziensis Germain et Evrard. Estudos sobre forrageiras na Amazônia, vol 2, nr 1. Instituto de Pesquisae Experimentação Agropecuária do Norte, Belém, Brazil

  • T Shimmen E Yokota (2004) ArticleTitleCytoplasmic streaming in plants Curr Opin Cell Biol 16 68–72 Occurrence Handle15037307 Occurrence Handle10.1016/j.ceb.2003.11.009 Occurrence Handle1:CAS:528:DC%2BD2cXitlSms7s%3D

    Article  PubMed  CAS  Google Scholar 

  • F Solomon (1987) ArticleTitleWhat myosin might do Science 236 1043–1044 Occurrence Handle3554513 Occurrence Handle10.1126/science.3554513 Occurrence Handle1:STN:280:DyaL2s3gs1ahsg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • VR Tilton (1981) ArticleTitleOvule development in Ornithogalum caudatum (Liliaceae) with a review of selected papers on angiosperm reproduction. IV. Egg apparatus structure and function New Phytol 88 505–531 Occurrence Handle10.1111/j.1469-8137.1981.tb04095.x

    Article  Google Scholar 

  • MR Tucker ACG Araujo N Paech V Hecht EDL Schmidt J-B Rossel SC De Vries AMG Koltunow (2003) ArticleTitleSexual and apomictic reproduction in Hieracium subgenus Pilosella are closely interrelated developmental pathways Plant Cell 15 1524–1537 Occurrence Handle12837944 Occurrence Handle10.1105/tpc.011742 Occurrence Handle1:CAS:528:DC%2BD3sXlslWktLc%3D

    Article  PubMed  CAS  Google Scholar 

  • JL van Went (1970) ArticleTitleThe ultrastructure of the synergids of Petunia Acta Bot Neerl 19 121–132

    Google Scholar 

  • JL van Went MTM Willemse (1984) Fertilization BM Johri (Eds) Embryology of angiosperms Springer Berlin Heidelberg New York 273–317

    Google Scholar 

  • J-P Vielle-Calzada ML Nuccio MA Budiman TL Thomas BL Burson MA Hussey RA Wing (1996) ArticleTitleComparative gene expression in sexual and apomictic ovaries of Pennisetum ciliari (L.) Link Plant Mol Biol 32 1085–1092 Occurrence Handle9002607 Occurrence Handle10.1007/BF00041392 Occurrence Handle1:CAS:528:DyaK2sXovFantg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • DP Wolf (1974) ArticleTitleThe cortical response in Xenopus laevis ova Dev Biol 40 102–115 Occurrence Handle4213423 Occurrence Handle10.1016/0012-1606(74)90112-2 Occurrence Handle1:CAS:528:DyaE2MXotlelug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • GM Yanochko AJ Yool (2002) ArticleTitleRegulated cationic channel function in Xenopus oocytes expressing Drosophila Big Brain J Neurosci 22 2530–2540 Occurrence Handle11923418 Occurrence Handle1:CAS:528:DC%2BD38XisF2gurk%3D

    PubMed  CAS  Google Scholar 

  • S Yumura H Mori Y Fukui (1984) ArticleTitleLocalization of actin and myosin for the study of ameboid movement in Dictyostelium using improved immunofluorescence J Cell Biol 99 894–899 Occurrence Handle6381508 Occurrence Handle10.1083/jcb.99.3.894 Occurrence Handle1:CAS:528:DyaL2cXlvVOls7o%3D

    Article  PubMed  CAS  Google Scholar 

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  1. Departamento de Biologia Celular, Universidade de Brasília, Brasília, Distrito Federal

    E. R. Alves & V. T. C. Carneiro

  2. Embrapa Recursos Genéticos e Biotecnologia, Empresa Brasileira de Pesquisa Agropecuária, Brasília, Distrito Federal

    E. R. Alves, V. T. C. Carneiro & D. M. A. Dusi

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  1. E. R. Alves
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  3. D. M. A. Dusi
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Correspondence to D. M. A. Dusi.

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Correspondence and reprints: Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Avenida W 5 Norte (Final), 70770-900 Brasília, DF, Brazil.

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Alves, E., Carneiro, V. & Dusi, D. In situ localization of three cDNA sequences associated with the later stages of aposporic embryo sac development of Brachiaria brizantha . Protoplasma 231, 161–171 (2007). https://doi.org/10.1007/s00709-007-0253-z

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