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Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds

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Summary.

Euterpe edulis Martius, a tropical palm species characterized as highly recalcitrant, accumulated dehydrin proteins in both the endosperm and the embryo of the mature seed, as detected by Western blot analysis and immunogold electron microscopy. Three major bands at molecular masses of approximately 16, 18, and 24 kDa were identified in both samples analysed. Immunogold electron microscopy studies detected the presence of dehydrins in the embryo and endosperm. In both cases, dehydrins were immunolocalized in cytoplasm and chromatin. No labelling associated with either membranes or organelles was detected. It is known that dehydrins are produced as part of the developmental program of orthodox seeds and are also present in some recalcitrant seeds of temperate regions. The constitutive presence of dehydrins in embryos of extremely recalcitrant species of tropical origin has not been previously reported.

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Authors and Affiliations

  1. Instituto de Recursos Biológicos, Centro de Investigación de Recursos Naturales, Instituto Nacional de Tecnología Agropecuria, Hurlingham, Buenos Aires

    V. Panza & S. Maldonado

  2. Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Buenos Aires

    V. Panza, P. Carjuzaa, V. Láinez & S. Maldonado

  3. Instituto de Biotecnología, Centro de Investigación de Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuria, Hurlingham, Buenos Aires

    A. J. Distéfano & M. del Vas

Authors
  1. V. Panza
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  2. A. J. Distéfano
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  3. P. Carjuzaa
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  4. V. Láinez
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  5. M. del Vas
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  6. S. Maldonado
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Correspondence to S. Maldonado.

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Correspondence and reprints: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, C1428EHA, Ciudad Autónoma de Buenos Aires, Argentina.

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Panza, V., Distéfano, A., Carjuzaa, P. et al. Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds. Protoplasma 231, 1–5 (2007). https://doi.org/10.1007/s00709-007-0248-9

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  • DOI: https://doi.org/10.1007/s00709-007-0248-9

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