Summary.
Chloroplasts alter their distribution within plant cells depending on the external light conditions. Myosin inhibitors 2,3-butanedione monoxime (BDM), N-ethylmaleimide (NEM), and 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML-7) were used to study the possible role of myosins in chloroplast photorelocation in Arabidopsis thaliana mesophyll cells. None of these agents had an effect on the chloroplast high-fluence-rate avoidance movement but all of the three myosin inhibitors blocked the accumulation movement of chloroplasts after a high-fluence-rate irradiation of the leaves. The results suggest that myosins have a role in A. thaliana chloroplast photorelocation.
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Abbreviations
- BDM:
-
2,3-butanedione monoxime
- ML-7:
-
1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride
- NEM:
-
N-ethylmaleimide
References
A Forer L Fabian (2005) ArticleTitleDoes 2,3-butanedione monoxime inhibit nonmuscle myosin? Protoplasma 225 1–4 Occurrence Handle15868207 Occurrence Handle10.1007/s00709-004-0077-z Occurrence Handle1:CAS:528:DC%2BD2MXjvVSqtb0%3D
K Hashimoto H Igarashi S Mano M Nishimura T Shimmen E Yokota (2005) ArticleTitlePeroxisomal localization of a myosin XI isoform in Arabidopsis thaliana Plant Cell Physiol 46 782–789 Occurrence Handle15792961 Occurrence Handle10.1093/pcp/pci085 Occurrence Handle1:CAS:528:DC%2BD2MXkvVWqurc%3D
C Holweg P Nick (2004) ArticleTitleArabidopsis myosin XI mutant is defective in organelle movement and polar auxin transport Proc Natl Acad Sci USA 101 10488–10493 Occurrence Handle15240891 Occurrence Handle10.1073/pnas.0403155101 Occurrence Handle1:CAS:528:DC%2BD2cXmtVyrsb4%3D
T Kagawa T Sakai N Suetsugu K Oikawa S Ishiguro T Kato S Tabata K Okada M Wada (2001) ArticleTitleArabidopsis NPL1: a phototropin homolog controlling the chloroplast high-light avoidance response Science 291 2138–2141 Occurrence Handle11251116 Occurrence Handle10.1126/science.291.5511.2138 Occurrence Handle1:CAS:528:DC%2BD3MXitFClsbc%3D
MK Kandasamy RB Meagher (1999) ArticleTitleActin-organelle interaction: association with chloroplast in Arabidopsis leaf mesophyll cells Cell Motil Cytoskeleton 44 110–118 Occurrence Handle10506746 Occurrence Handle10.1002/(SICI)1097-0169(199910)44:2<110::AID-CM3>3.0.CO;2-O Occurrence Handle1:STN:280:DyaK1Mvjsl2msQ%3D%3D
M Kasahara T Kagawa K Oikawa N Suetsugu M Miyao M Wada (2002) ArticleTitleChloroplast avoidance movement reduces photodamage in plants Nature 420 829–832 Occurrence Handle12490952 Occurrence Handle10.1038/nature01213 Occurrence Handle1:CAS:528:DC%2BD38XpsFygt7o%3D
S Liebe D Menzel (1995) ArticleTitleActomyosin-based motility of endoplasmic reticulum and chloroplasts in Vallisneria mesophyll cells Biol Cell 85 207–222 Occurrence Handle8785522 Occurrence Handle10.1016/0248-4900(96)85282-8 Occurrence Handle1:CAS:528:DyaK28Xkt1Sru74%3D
DW McCurdy (1999) ArticleTitleIs 2,3-butanedione monoxime an effective inhibitor of myosin-based activities in plant cells? Protoplasma 209 120–125 Occurrence Handle10.1007/BF01415707 Occurrence Handle1:CAS:528:DC%2BD3cXitVeqsLo%3D
K Oikawa M Kasahara T Kiyosue T Kagawa N Suetsugu F Takahashi T Kanegae Y Niwa A Kadota M Wada (2003) ArticleTitleCHLOROPLAST UNUSUAL POSITIONING1 is essential for proper chloroplast positioning Plant Cell 15 2805–2815 Occurrence Handle14615600 Occurrence Handle10.1105/tpc.016428 Occurrence Handle1:CAS:528:DC%2BD2cXhtVSitA%3D%3D
E Ostap (2002) ArticleTitle2,3-Butanedione monoxime (BDM) as a myosin inhibitor J Muscle Res Cell Motil 23 305–308 Occurrence Handle12630704 Occurrence Handle10.1023/A:1022047102064 Occurrence Handle1:CAS:528:DC%2BD3sXkvFejuw%3D%3D
AS Reddy IS Day (2001) ArticleTitleAnalysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence Genome Biol 2 research0024 Occurrence Handle11516337 Occurrence Handle10.1186/gb-2001-2-7-research0024 Occurrence Handle1:CAS:528:DC%2BD38Xit1ChsrY%3D
T Shimmen E Yokota (2004) ArticleTitleCytoplasmic streaming in plants Curr Opin Cell Biol 16 68–72 Occurrence Handle15037307 Occurrence Handle10.1016/j.ceb.2003.11.009 Occurrence Handle1:CAS:528:DC%2BD2cXitlSms7s%3D
H Sugi S Chaen (2003) ArticleTitleForce-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells J Exp Biol 206 1971–1976 Occurrence Handle12756278 Occurrence Handle10.1242/jeb.00239 Occurrence Handle1:CAS:528:DC%2BD3sXlslaltLo%3D
M Wada T Kagawa Y Sato (2003) ArticleTitleChloroplast movement Annu Rev Plant Biol 54 455–468 Occurrence Handle14502999 Occurrence Handle10.1146/annurev.arplant.54.031902.135023 Occurrence Handle1:CAS:528:DC%2BD3sXntFSnsr0%3D
Z Wang TC Pesacreta (2004) ArticleTitleA subclass of myosin XI is associated with mitochondria, plastids, and the molecular chaperone subunit TCP-1α in maize Cell Motil Cytoskeleton 57 218–232 Occurrence Handle14752806 Occurrence Handle10.1002/cm.10168 Occurrence Handle1:CAS:528:DC%2BD2cXjslyhtb8%3D
GO Wasteneys J Willingale-Theune D Menzel (1997) ArticleTitleFreeze shattering: a simple and effective method for permeabilizing higher plant cell walls J Microsc 188 51–61 Occurrence Handle9369020 Occurrence Handle10.1046/j.1365-2818.1977.2390796.x Occurrence Handle1:STN:280:DyaK1c%2FjsFGjug%3D%3D
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Correspondence and reprints: Department of Gene Technology, Tallinn, University of Technology, Akadeemia tee 15, 19086 Tallinn, Estonia.
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Paves, H., Truve, E. Myosin inhibitors block accumulation movement of chloroplasts in Arabidopsis thaliana leaf cells. Protoplasma 230, 165–169 (2007). https://doi.org/10.1007/s00709-006-0230-y
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DOI: https://doi.org/10.1007/s00709-006-0230-y