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Comparison of the toxicity and distribution of cadmium and lead in plant cells

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Summary.

The toxicity of heavy metals (Cd, Zn, and Pb) was assessed by in vivo observations of their effect on cytoplasmic streaming in Allium cepa L. bulb scale epidermal cells. On the basis of our results, the order of toxicity of the studied cations is Zn < Pb ≪ Cd. The difference in toxicity between cadmium and lead was found to be very large. When cytoplasmic streaming was assessed, this difference was threefold. When the total content of cadmium and lead (determined by inductively coupled plasma mass spectrometry) was the criterion, the difference in toxicity was 15-fold. Fractionation of the tissue and enzymatic digestion of the cells revealed that the largest proportion of cadmium was located in the cell walls (56%), whereas almost all of the lead (97.6%) was accumulated in an insoluble form. The speciation of water-soluble Pb and Cd fractions is discussed on the basis of analysis by capillary zone electrophoresis interfaced with inductively coupled plasma mass spectrometry of water extracts from epidermal cells. Lead and cadmium appeared to be bound mainly to salts, which explains their toxicity. Cadmium was complexed (detoxified) by organic acids, while thiols were the metal-complexing species for lead. Histidine formed complexes with both cadmium and lead. Ultrastructural analyses showed that lead was encapsulated in small vesicles in the cytoplasm. Fluorescence studies of the endoplasmic reticulum (ER) revealed that it underwent extensive fragmentation under the influence of lead, with numerous ER vesicles appearing in the cells. In other words, the lead deposits in the cytoplasm were contained in vesicles arising from fragmentation of the ER. These observations indicate that epidermal cells have a rapid and effective mechanism for detoxifying lead involving the ER, and this may be one of the mechanisms accounting for the lower toxicity of lead in comparison with cadmium. The suitability of Allium cepa bulb scale epidermal cells for use in ecotoxicological studies is also discussed. Step-by-step directions for this test are given.

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Abbreviations

CZE-ICP-MS:

capillary zone electrophoresis interfaced with inductively coupled plasma mass spectrometry

DiOC6 :

3,3′-dihexyloxacarbocyanine iodide

ER:

endoplasmic reticulum

ICP-MS:

inductively coupled plasma mass spectrometry

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Authors and Affiliations

  1. Department of Ecotoxicology, Institute of Experimental Plant Biology, Faculty of Biology, University of Warsaw, Warsaw

    M. H. Wierzbicka & E. Przedpełska

  2. Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Warsaw

    R. Ruzik, K. Połeć-Pawlak & M. Jarosz

  3. Laboratory of Bioinorganic Analytical Chemistry, Unité Mixte Recherche 5034, Centre National de la Recherche Scientifique, Université de Pau et des Pays de l’Adour, Pau

    L. Ouerdane & J. Szpunar

  4. Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Warsaw

    A. Szakiel

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  1. M. H. Wierzbicka
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  2. E. Przedpełska
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  3. R. Ruzik
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  4. L. Ouerdane
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  5. K. Połeć-Pawlak
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  6. M. Jarosz
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  7. J. Szpunar
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  8. A. Szakiel
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Correspondence to M. H. Wierzbicka.

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Correspondence and reprints: Department of Ecotoxicology, Institute of Experimental Plant Biology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.

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Wierzbicka, M., Przedpełska, E., Ruzik, R. et al. Comparison of the toxicity and distribution of cadmium and lead in plant cells. Protoplasma 231, 99–111 (2007). https://doi.org/10.1007/s00709-006-0227-6

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  • DOI: https://doi.org/10.1007/s00709-006-0227-6

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