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Effect of leaf position on levels of the chloroplast and cytosolic fructose bisphosphatase isozymes in the pea leaf nucleus

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Summary.

Immunocytolocalization experiments indicate that nuclear levels of the pea leaf cytosolic fructose bisphosphatase are higher in leaves located near the base of the plant and lower in expanded leaves at the apex. It seems possible that the cytosolic isozyme in the nucleus has a role in tissue aging. In contrast, there is no indication that leaf position or tissue age affects levels of the chloroplastic enzyme in the nucleus. The density of the chloroplast fructose bisphosphatase is higher in the nucleolus than in the nucleoplasm. Conversely, the density of the cytosolic isozyme is slightly higher in the nucleoplasm. Analysis of double immunolabeling experiments indicates that both isozymes are distributed nonrandomly with respect to DNA, and therefore colocalized with DNA, in the nucleus, and that the chloroplast isozyme is also distributed nonrandomly with respect to DNA in the chloroplast.

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References

  • JB Anderson AA Carol VK Brown LE Anderson (2003) ArticleTitleA quantitative method for assessing co-localization in immuno-labeled thin section electron micrographs J Struct Biol 143 95–106 Occurrence Handle12972346 Occurrence Handle10.1016/S1047-8477(03)00138-2 Occurrence Handle1:CAS:528:DC%2BD3sXntVGltbg%3D

    Article  PubMed  CAS  Google Scholar 

  • LE Anderson AA Carol (2005) ArticleTitleEnzyme co-localization in the pea leaf cytosol: 3-P-glycerate kinase, glyceraldehyde-3-P dehydrogenase, triose-P isomerase and aldolase Plant Sci 169 620–628 Occurrence Handle10.1016/j.plantsci.2005.05.012 Occurrence Handle1:CAS:528:DC%2BD2MXmsVOhtrg%3D

    Article  CAS  Google Scholar 

  • LE Anderson X Wang JT Gibbons (1995) ArticleTitleThree enzymes of carbon metabolism, or their antigenic analogs, in pea nuclei Plant Physiol 108 659–667 Occurrence Handle7610163 Occurrence Handle10.1104/pp.108.2.659 Occurrence Handle1:CAS:528:DyaK2MXmtlaqsrc%3D

    Article  PubMed  CAS  Google Scholar 

  • LE Anderson R Yousefzai MR Ringenberg AA Carol (2004a) ArticleTitleBoth chloroplastic and cytosolic fructose bisphosphatase isozymes are present in the pea leaf nucleus Plant Sci 166 721–730 Occurrence Handle10.1016/j.plantsci.2003.11.008 Occurrence Handle1:CAS:528:DC%2BD2cXhtFWgsbc%3D

    Article  CAS  Google Scholar 

  • LE Anderson MR Ringenberg AA Carol (2004b) ArticleTitleCytosolic glyceraldehyde-3-P dehydrogenase and the B subunit of the chloroplast enzyme are present in the pea leaf nucleus Protoplasma 223 33–43 Occurrence Handle10.1007/s00709-003-0030-6 Occurrence Handle1:CAS:528:DC%2BD2cXhsl2nsrs%3D

    Article  CAS  Google Scholar 

  • LE Anderson JA Bryant AA Carol (2004c) ArticleTitleBoth chloroplastic and cytosolic phosphoglycerate kinase isozymes are present in the pea leaf nucleus Protoplasma 223 103–110 Occurrence Handle1:CAS:528:DC%2BD2cXltVKntLo%3D

    CAS  Google Scholar 

  • LE Anderson MR Ringenberg VK Brown AA Carol (2005a) ArticleTitleBoth chloroplastic and cytosolic phosphofructoaldolase isozymes are present in the pea leaf nucleus Protoplasma 225 235–242 Occurrence Handle10.1007/s00709-005-0099-1 Occurrence Handle1:CAS:528:DC%2BD2MXhtFWmtLbI

    Article  CAS  Google Scholar 

  • LE Anderson N Gatla AA Carol (2005b) ArticleTitleEnzyme co-localization in pea leaf chloroplasts: glyceraldehyde-3-P dehydrogenase, triose-P isomerase, aldolase and sedoheptulose bisphosphatase Photosynth Res 83 317–328 Occurrence Handle10.1007/s11120-005-0790-2 Occurrence Handle1:CAS:528:DC%2BD2MXjtlKlsLs%3D

    Article  CAS  Google Scholar 

  • LE Anderson AA Carol JT Gibbons (2006) ArticleTitleEffect of leaf position on levels of the chloroplast and cytosolic phosphofructoaldolase isozymes in the pea leaf nucleus Int J Plant Sci 167 257–268 Occurrence Handle10.1086/498694 Occurrence Handle1:CAS:528:DC%2BD28XjtFejtro%3D

    Article  CAS  Google Scholar 

  • MD Berry AA Boulton (2000) ArticleTitleGlyceraldehyde-3-phosphate dehydrogenase and apoptosis J Neurosci Res 60 150–154 Occurrence Handle10740219 Occurrence Handle10.1002/(SICI)1097-4547(20000415)60:2<150::AID-JNR3>3.0.CO;2-4 Occurrence Handle1:CAS:528:DC%2BD3cXisVWjt7k%3D

    Article  PubMed  CAS  Google Scholar 

  • A Gizak A Dzugaj (2003) ArticleTitleFBPase is in the nuclei of cardiomyocytes FEBS Lett 539 51–55 Occurrence Handle12650925 Occurrence Handle10.1016/S0014-5793(03)00183-2 Occurrence Handle1:CAS:528:DC%2BD3sXitFams70%3D

    Article  PubMed  CAS  Google Scholar 

  • A Gizak D Rakus A Dzugaj (2005) ArticleTitleNuclear localization of fructose 1,6-bisphosphatase in smooth muscle cells J Mol Histol 36 243–248 Occurrence Handle16200456 Occurrence Handle10.1007/s10735-005-6523-1 Occurrence Handle1:CAS:528:DC%2BD2MXhtVKit7rJ

    Article  PubMed  CAS  Google Scholar 

  • R Hermoso MR Defelipe A Vivo A Chueca JJ Lazaro JL Gorge (1989) ArticleTitleImmunogold localization of photosynthetic fructose-1,6-bisphosphatase in pea leaf tissue Plant Physiol 89 381–385 Occurrence Handle16666543 Occurrence Handle1:CAS:528:DyaL1MXhtFWrsr8%3D Occurrence Handle10.1104/pp.89.1.381

    Article  PubMed  CAS  Google Scholar 

  • JC Jang P Leon L Zhou J Sheen (1997) ArticleTitleHexokinase as a sugar sensor in higher plants Plant Cell 9 5–19 Occurrence Handle9014361 Occurrence Handle10.1105/tpc.9.1.5 Occurrence Handle1:CAS:528:DyaK2sXot1Gjsg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • PA Saunders R-W Chen D-M Chuang (1999) ArticleTitleNuclear translocation of glyceraldehyde-3-phosphate dehydrogenase isoforms during neuronal apoptosis J Neurochem 72 925–932 Occurrence Handle10037463 Occurrence Handle10.1046/j.1471-4159.1999.0720925.x Occurrence Handle1:CAS:528:DyaK1MXhsFWgsr8%3D

    Article  PubMed  CAS  Google Scholar 

  • A Sawa AA Khan LD Hester SH Snyder (1997) ArticleTitleGlyceraldehyde-3-phosphate dehydrogenase: nuclear translocation participates in neuronal and nonneuronal cell death Proc Natl Acad Sci USA 94 11669–11674 Occurrence Handle9326668 Occurrence Handle10.1073/pnas.94.21.11669 Occurrence Handle1:CAS:528:DyaK2sXmslejt7g%3D

    Article  PubMed  CAS  Google Scholar 

  • HD Schmitz (2001) ArticleTitleReversible nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase upon serum depletion Eur J Cell Biol 80 419–427 Occurrence Handle11484933 Occurrence Handle10.1078/0171-9335-00174 Occurrence Handle1:CAS:528:DC%2BD3MXmtVOrtLg%3D

    Article  PubMed  CAS  Google Scholar 

  • AJ Yáñez R Bertinat II Concha JC Slebe (2003) ArticleTitleNuclear localization of liver FBPase isoenzyme in kidney and liver FEBS Lett 550 35–40 Occurrence Handle12935882 Occurrence Handle10.1016/S0014-5793(03)00809-3 Occurrence Handle1:CAS:528:DC%2BD3sXmsVKnsbs%3D

    Article  PubMed  CAS  Google Scholar 

  • AJ Yáñez M Garcia-Rocha R Bertinat C Droppelmann II Concha JJ Guinovart JC Slebe (2004) ArticleTitleSubcellular localization of liver FBPase is modulated by metabolic conditions FEBS Lett 577 154–158 Occurrence Handle15527777 Occurrence Handle10.1016/j.febslet.2004.09.077 Occurrence Handle1:CAS:528:DC%2BD2cXpsFersr0%3D

    Article  PubMed  CAS  Google Scholar 

  • M Zmojdzian D Dziewulska-Szwajkowska A Dzugaj (2005) ArticleTitleLocalization of chicken muscle FBPase in cardiomyocyte nuclei Comp Biochem Physiol B Biochem Mol Biol 140 37–43 Occurrence Handle15621507 Occurrence Handle10.1016/j.cbpc.2004.04.016 Occurrence Handle1:CAS:528:DC%2BD2MXhtVClsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, University of Illinois-Chicago, Chicago, Illinois

    L. E. Anderson & J. T. Gibbons

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  1. L. E. Anderson
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  2. J. T. Gibbons
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Correspondence to L. E. Anderson.

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Correspondence and reprints: Department of Biological Sciences m/c 066, University of Illinois-Chicago, 845 West Taylor, Chicago, Illinois 60607-7060, U.S.A.

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Anderson, L., Gibbons, J. Effect of leaf position on levels of the chloroplast and cytosolic fructose bisphosphatase isozymes in the pea leaf nucleus. Protoplasma 231, 113–121 (2007). https://doi.org/10.1007/s00709-006-0224-9

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  • DOI: https://doi.org/10.1007/s00709-006-0224-9

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