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Localisation of fucoxanthin chlorophyll a/c-binding polypeptides of the centric diatom Cyclotella cryptica by immuno-electron microscopy

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Summary.

Antisera were raised against the C termini of three fucoxanthin chlorophyll a/c-binding polypeptides, Fcp2, Fcp4, and Fcp6, of the centric diatom Cyclotella cryptica. Immunogold electron microscopy of ultrathin-sectioned cells indicated that Fcp2 and Fcp4 are present in almost the same amounts, whereas approximately 8- to 10-fold less gold label was registered for Fcp6. Immunogold electron microscopy of freeze-fracture replicas of thylakoid membranes showed that the C termini of at least Fcp2 and Fcp4 were located in the thylakoid lumen, thus demonstrating a 3-dimensional structure similar to that already described for the chlorophyll a/b-binding light-harvesting polypeptides of higher plants.

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Abbreviations

Chl:

chlorophyll

Fcp:

fucoxanthin chlorophyll a/c-binding polypeptide

LHC:

light-harvesting complex

PS I:

photosystem I

PS II:

photosystem II

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Authors and Affiliations

  1. Elektronenmikroskopisches Zentrum, Klinikum der Friedrich-Schiller-Universität Jena, Jena

    M. Westermann

  2. Arbeitsgruppe Geomikrobiologie, Institut für Chemie und Biologie des Meeres, Carl-von-Ossietzky-Universität Oldenburg, Oldenburg

    E. Rhiel

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  1. M. Westermann
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  2. E. Rhiel
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Correspondence and reprints: Elektronenmikroskopisches Zentrum, Klinikum der Friedrich-Schiller-Universität Jena, Ziegelmühlenweg 1, 07743 Jena, Federal Republic of Germany.

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Westermann, M., Rhiel, E. Localisation of fucoxanthin chlorophyll a/c-binding polypeptides of the centric diatom Cyclotella cryptica by immuno-electron microscopy. Protoplasma 225, 217–223 (2005). https://doi.org/10.1007/s00709-005-0083-9

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  • DOI: https://doi.org/10.1007/s00709-005-0083-9

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