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Effect of the type of commercially available mixed-mode stationary phases on the retention of pharmaceutically important acidic and neutral compounds using different compositions of mobile phase

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Abstract

Four different columns with mixed-mode properties were used to investigate the retention behavior of four acidic compounds (ascorbic, nicotinic, p-toluenesulfonic, and p-hydroxybenzenesulfonic acids) and two neutral compounds (dexamethasone and paracetamol). The effect of mobile phase composition (acetonitrile content and buffer pH) on the retention of the analytes was studied to better understand the separation role of an ion-exchange group position within a stationary phase for acidic analytes. The impact of C18 hydrophobic moiety, identical for all the tested stationary phases, was studied on retention of two neutral compounds via selected parameters characterizing the investigated stationary phases. The results obtained were compared and discussed with data previously reached using an Acclaim™ WAX-1 mixed-mode column.

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Acknowledgements

This work was financed by the Grant Agency of the Charles University, Czech Republic, project SVV 260560.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic

    Sofiya Grybinik & Zuzana Bosakova

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  1. Sofiya Grybinik
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  2. Zuzana Bosakova
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Correspondence to Zuzana Bosakova.

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Grybinik, S., Bosakova, Z. Effect of the type of commercially available mixed-mode stationary phases on the retention of pharmaceutically important acidic and neutral compounds using different compositions of mobile phase. Monatsh Chem 153, 719–725 (2022). https://doi.org/10.1007/s00706-022-02960-6

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  • DOI: https://doi.org/10.1007/s00706-022-02960-6

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