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Capillary monolithic columns based on poly(styrene-divinylbenzene-methacrylic acid) copolymer for liquid chromatography and electrochromatography

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Abstract

This work deals with newly developed poly(styrene-divinylbenzene-methacrylic acid) monolithic columns for capillary electrochromatographic and capillary liquid chromatographic separations of low-molecular-weight organic compounds. The influence of the polymerization time on chromatographic properties of prepared monolithic capillary columns was studied by capillary electrochromatography and capillary liquid chromatography using a model mixture of thiourea, benzene, and its homologues. The effect of electrostatic interactions between methacrylic acid as a weak cation-exchanger and selected analytes (a mixture of three amino acids, and thiourea, or diethylamine) on their retention in capillary liquid chromatography was investigated. The determined run-to-run and batch-to-batch repeatabilities indicate high reliability of the preparation of the separation media.

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References

  1. Švec F, Fréchet JMJ (1992) Anal Chem 64:820

    Article  Google Scholar 

  2. Švec F (2017) Electrophoresis 38:2810

    Article  Google Scholar 

  3. Švec F (2004) J Sep Sci 27:1419

    Article  Google Scholar 

  4. Meyers JJ, Liapis AI (1999) J Chromatogr A 852:3

    Article  CAS  Google Scholar 

  5. Vojta J, Musilová-Svobodová A, Franc M, Coufal P, Bosáková Z (2014) Chem Listy 108:127

    CAS  Google Scholar 

  6. Palm A, Novotný MV (1997) Anal Chem 69:4499

    Article  CAS  Google Scholar 

  7. Fujimoto C, Kino J, Sawada H (1995) J Chromatogr A 716:107

    Article  CAS  Google Scholar 

  8. Gusev I, Huang X, Horváth C (1999) J Chromatogr A 855:273

    Article  CAS  Google Scholar 

  9. Petro M, Švec F, Gitsov I, Fréchet JMJ (1996) Anal Chem 68:315

    Article  CAS  Google Scholar 

  10. Grafnetter J, Coufal P, Tesařová E, Suchánková J, Bosáková Z, Ševčík J (2004) J Chromatogr A 1049:43

    Article  CAS  Google Scholar 

  11. Buszewski B, Szumski M (2004) Chromatographia 60:S261

    Article  CAS  Google Scholar 

  12. Mayr B, Hölzl G, Eder K, Buchmeiser MR, Huber CG (2002) Anal Chem 74:6080

    Article  CAS  Google Scholar 

  13. Huang X, Zhang J, Horváth C (1999) J Chromatogr A 858:91

    Article  CAS  Google Scholar 

  14. Oberacher H, Huber CG (2002) Trends Anal Chem 21:166

    Article  CAS  Google Scholar 

  15. Ericson C, Liao JL, Nakazato K, Hjérten S (1997) J Chromatogr A 767:33

    Article  CAS  Google Scholar 

  16. Kučerová Z, Szumski M, Buszewski B, Jandera P (2007) J Sep Sci 30:3018

    Article  Google Scholar 

  17. Jin WH, Fu HJ, Huang XD, Xiao H, Zou HF (2003) Electrophoresis 24:3172

    Article  CAS  Google Scholar 

  18. Xiong BH, Zhang LH, Zhang YK, Zou HF, Wang JD (2000) J High Resolut Chromatogr 23:67

    Article  CAS  Google Scholar 

  19. Currivan S, Jandera P (2014) Chromatography 1:24

    Article  Google Scholar 

  20. Huang HY, Lin HY, Lin SP (2006) Electrophoresis 27:4674

    Article  CAS  Google Scholar 

  21. Nordborg A, Hilder EF, Haddad PR (2011) Annu Rev Anal Chem 4:197

    Article  CAS  Google Scholar 

  22. Lynch KB, Ren JT, Beckner MA, He CY, Liu SR (2019) Anal Chim Acta 1046:48

    Article  CAS  Google Scholar 

  23. Eelting S, Wouters S, Dores-Sousa JL, Švec F (2017) J Chromatogr A 1498:8

    Article  Google Scholar 

  24. Švec F (2012) J Chromatogr A 1228:250

    Article  Google Scholar 

  25. Svobodová A, Křížek T, Širc J, Šálek P, Tesařová E, Coufal P, Štulík K (2011) J Chromatogr A 1218:1544

    Article  Google Scholar 

  26. Courtois J, Szumski M, Byström E, Iwasiewicz A, Shchukarev A, Irgum K (2006) J Sep Sci 29:14

    Article  CAS  Google Scholar 

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Acknowledgements

The authors gratefully acknowledge Charles University, Project SVV260560, for the financial support.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Charles University, Faculty of Science, Albertov 6, 128 43, Prague, Czech Republic

    Adéla Musilová, Jiří Vojta, Tomáš Křížek, Anna Kubíčková & Pavel Coufal

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  1. Adéla Musilová
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  2. Jiří Vojta
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  3. Tomáš Křížek
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  4. Anna Kubíčková
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  5. Pavel Coufal
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Correspondence to Anna Kubíčková.

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Musilová, A., Vojta, J., Křížek, T. et al. Capillary monolithic columns based on poly(styrene-divinylbenzene-methacrylic acid) copolymer for liquid chromatography and electrochromatography. Monatsh Chem 153, 759–765 (2022). https://doi.org/10.1007/s00706-022-02944-6

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  • DOI: https://doi.org/10.1007/s00706-022-02944-6

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