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Detection and identification of engineered nanoparticles in exhaled breath condensate, blood serum, and urine of occupationally exposed subjects

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Abstract

The use of nanotechnology and the fields of application of nanomaterials are growing vastly, but the negative health effects on the exposed employees are not well studied. The standardized methods of monitoring of occupational exposure are still absent. The task of occupational physicians is to find the ways of evaluation of potential risks of exposure to engineered nanoparticles and to determine the biomarkers for early diagnostics and prevention of occupational diseases. The aim of our study was to detect and identify engineered nanoparticles in biological samples received from occupationally exposed subjects and to evaluate the association of findings with the results of external aerosol measurements on the workplace. The study cohort consisted of two groups of subjects. The first group was exposed to engineered nanoparticles containing mainly iron, manganese, and carbon compounds; the second group was exposed to the nanoparticles containing copper oxide. The post-shift biological samples (urine, blood serum, and exhaled breath condensate) were collected. The analysis was performed by transmission electron microscopy and energy-dispersive spectroscopy. The nanoparticles were detected in all the biological samples. The most common identified chemical elements were the biogenic ones (carbon, potassium, chlorine, oxygen), but the nanoparticles containing metals were identified in EBC, blood, and urine as well (gold, silver, copper, lanthanum, cerium, and tantalum). Our results demonstrate the possibility of detection of occupational exposure to the engineered nanoparticles in human biological fluids. Further studies are necessary to compare the pre-shift and post-shift burden of samples with engineered nanoparticles and to determine the magnitude of occupational exposure during the shift.

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Acknowledgements

This study was supported by the Czech Science Foundation (GA ČR No. 18-02079S), Czech Science Foundation Grant no. P503/12/G147, the Projects PROGRES Q25, Q29 of Charles University in Prague and project GAUK of Charles University in Prague (No. 192718).

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Authors and Affiliations

  1. Department of Occupational Medicine, First Faculty of Medicine, Charles University, Na Bojišti 1, 120 00, Prague, Czech Republic

    Lucie Lischkova, Daniela Pelclova, Jiri Hlusicka, Stepanka Vlckova, Zdenka Fenclova & Sergey Zakharov

  2. Toxicological Information Centre, General University Hospital, Na Bojišti 1, 120 00, Prague, Czech Republic

    Lucie Lischkova, Daniela Pelclova, Jiri Hlusicka, Zdenka Fenclova & Sergey Zakharov

  3. J. Heyrovsky Institute of Physical Chemistry of the Czech Academy of Sciences, Dolejškova 3, 182 23, Prague, Czech Republic

    Tomas Navratil

  4. Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Kateřinská 32, 121 08, Prague 2, Czech Republic

    Tomas Navratil

  5. Faculty of Mechanical Engineering, Department of Machining and Assembly, Department of Engineering Technology, Department of Material Science, Technical University of Liberec, Studentská 1402/2, 461 17, Liberec, Czech Republic

    Stepanka Dvorackova & Alexey Popov

  6. Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Technická 5, 166 28, Prague 6, Czech Republic

    Alena Michalcova & Ivo Marek

  7. Institute of Analytical Chemistry of the CAS, Veveří 97, 602 00, Brno, Czech Republic

    Pavel Mikuska

  8. Institute of Chemical Process Fundamentals of the CAS, Rozvojová 1/135, 165 02, Prague 6, Czech Republic

    Vladimir Zdimal, Martin Kostejn, Jakub Ondracek & Jaroslav Schwarz

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  1. Lucie Lischkova
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  2. Daniela Pelclova
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Correspondence to Lucie Lischkova.

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Lischkova, L., Pelclova, D., Hlusicka, J. et al. Detection and identification of engineered nanoparticles in exhaled breath condensate, blood serum, and urine of occupationally exposed subjects. Monatsh Chem 150, 511–523 (2019). https://doi.org/10.1007/s00706-019-2379-z

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  • DOI: https://doi.org/10.1007/s00706-019-2379-z

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