Abstract
The paper presents the findings of an optimization and validation results of electrochemical detection of 8-hydroxyguanine, a major marker of oxidative stress, using disposable screen-printed carbon electrode (SPCE) modified with carboxy-functionalized multi-walled carbon nanotubes (MWCNTs-COOH). Several types of bare and modified SPCEs were tested and modification with MWCNTs-COOH was found superior in terms of measured current response, level of background currents, and sensitivity in oxidation of 8-hydroxyguanine. The effect of pH and composition of supporting electrolyte on current response and peak potential were also evaluated. Validation parameters were calculated for optimum pH 7 of 0.01 mol dm−3 phosphate buffer. The sensor showed the highest sensitivity of 0.0625 µA dm3 μmol−1 within the linear range 0.3–12 μmol dm−3 with a limit of detection as low as 0.57 μmol dm−3. Extended linear range 0.3–240 μmol dm−3 is feasible with a sensitivity of 0.0523 µA dm3 μmol−1. Intra-day and inter-day assays within 3 days were performed to verify the repeatability of modified screen-printed carbon sensors. Finally, the sensor was utilized for electrochemical monitoring of stability of 8-hydroxyguanine when stored in room temperature, in the refrigerator, or frozen and thawed.
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References
Salmon TB, Evert BA, Song B, Doetsch PW (2004) Nucleic Acids Res 32:3712
Dizdaroglu M, Jaruga P, Birincioglu M, Rodriguez H (2002) Free Radic Biol Med 32:1102
Breen AP, Murphy JA (1995) Free Radic Biol Med 18:1033
Dizdaroglu M, Jaruga P (2012) Free Radic Res 46:382
Goyal RN, Bishnoi S (2010) Biosens Bioelectron 26:463
Kawai K, Kasai H, Li YS, Kawasaki Y, Watanabe S, Ohta M, Yamato H (2018) Genes Environ 40:5
Lu AL, Li X, Gu Y, Wright PM, Chang DY (2001) Cell Biochem Biophys 35:141
Bishnoi S, Goyal RN, Shim YB (2014) Bioelectrochemistry 99:24
Loft S, Svoboda P, Kawai K, Kasai H, Sørensen M, Tjønneland A, Vogel U, Møller P, Overvad K, Raaschou-Nielsen O (2012) Free Radic Biol Med 52:167
Dizdaroglu M (1985) Biochemistry 24:4476
Hu CW, Cooke MS, Tsai YH, Chao MR (2015) Arch Toxicol 89:201
Andreoli R, Manini P, Palma GD, Alinovi R, Goldoni M, Niessen WM, Mutti A (2010) Biomarkers 15:221
Zitka O, Krizkova S, Skalickova S, Kopel P, Babula P, Adam V, Kizek R (2013) Comb Chem High Throughput Screen 16:130
Oliveira Brett AM, Piedade JAP, Serrano SHP (2000) Electroanalysis 12:969
Rebelo I, Piedade JAP, Oliveira Brett AM (2004) Bioelectrochemistry 63:267
Bernalte E, Carroll M, Banks CE (2017) Sens Actuators B Chem 247:896
Bioanalytical method validation: guidance for industry (2018) https://www.fda.gov/downloads/Drugs/Guidances/ucm070107.pdf. Accessed 13 Dec 2018
Acknowledgements
This work was supported by the Ministry of Defense of the Czech Republic (Long-term organization development plan Medical Aspects of Weapons of Mass Destruction of the Faculty of Military Health Sciences, University of Defense) and by the Ministry of Education, Youth and Sport of the Czech Republic (Specific research project no: FVZ 201602).
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Jeličová, M., Metelka, R., Pejchal, J. et al. Electrochemical detection of 8-hydroxyguanine using screen-printed carbon electrodes modified with carboxy-functionalized multi-walled carbon nanotubes. Monatsh Chem 150, 1187–1193 (2019). https://doi.org/10.1007/s00706-019-02433-3
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DOI: https://doi.org/10.1007/s00706-019-02433-3