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Chlorpyrifos-methyl oxon hydrolysis and its monitoring by HPLC–MS/MS

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Abstract

Organophosphates belong to a family of acetylcholinesterase inhibiting neurotoxins. The toxicity of the parent compound, e.g., chlorpyrifos-methyl is usually tested. However, the toxicity of its transformation products and the transformation process itself are often overlooked. The chlorpyrifos-methyl oxon degradation reaction was monitored using the newly developed, reversed phase high-performance liquid chromatography methods with tandem mass spectrometry detection. These methods were applied to examine chlorpyrifos-methyl oxon hydrolysis at pH 9.2 and at 40 °C, thereby determining the two hydrolysis products in a single, 6-min run without internal standards. The method is cheaper and less demanding than previous methods, particularly regarding sample preparation.

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Acknowledgements

We gratefully acknowledge the support of the Charles University (SVV project no. 260440). This study was also partly funded by a grant from the Minority Serving Institutions Science, Technology, Engineering, and Mathematics Research & Development Corporation (MSRDC) Grant # D01_W911SR-14-2-0001-0008. We thank Carlos V. Melo for proofreading the manuscript.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, The City College of New York, 160 Convent Avenue, New York, NY, 10031, USA

    Ondřej Hodek, Urs Jans & Lijia Yang

  2. Ph.D. Program in Chemistry, Graduate Center of the City University of New York, New York, NY, 10016, USA

    Ondřej Hodek, Urs Jans & Lijia Yang

  3. Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 2030/8, 128 00, Prague 2, Czech Republic

    Ondřej Hodek & Tomáš Křížek

Authors
  1. Ondřej Hodek
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  2. Urs Jans
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  3. Lijia Yang
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  4. Tomáš Křížek
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Correspondence to Ondřej Hodek.

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Hodek, O., Jans, U., Yang, L. et al. Chlorpyrifos-methyl oxon hydrolysis and its monitoring by HPLC–MS/MS. Monatsh Chem 149, 1515–1519 (2018). https://doi.org/10.1007/s00706-018-2219-6

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  • DOI: https://doi.org/10.1007/s00706-018-2219-6

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