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New assay of α-l-rhamnosidase

Abstract

Free rutinose was prepared by enzymatic hydrolysis of rutin using defatted seed meal from tartary buckwheat. This disaccharide was used as substrate in spectrophotometric assay of α-l-rhamnosidase. The assay is based on hydrolysis of rutinose and subsequent determination of released glucose by a standard glucose oxidase assay kit. The method is easy to perform and requires no expensive equipment. The assay was applied in α-l-rhamnosidase estimation in ten commercial enzyme preparations and compared with standard assay on chromogenic substrate.

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Acknowledgements

This work was supported by the Slovak Research and Development Agency under the contract no. APVV-0846-12, the Scientific Grant Agency of the Ministry of Education of Slovak Republic, and Slovak Academy of Sciences (VEGA) under the project no. 2/0157/16 and also the Research and Development Operational Programmes funded by the ERDF (“Centre of Excellence on Green Chemistry Methods and Processes”, CEGreenI, contract no. 26240120001, as well as the “Amplification of the Centre of Excellence on Green Chemistry Methods and Processes”, CEGreenII, contract no. 26240120025).

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Correspondence to Vladimír Mastihuba.

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Karnišová Potocká, E., Mastihubová, M., Čičová, I. et al. New assay of α-l-rhamnosidase. Monatsh Chem 149, 167–174 (2018). https://doi.org/10.1007/s00706-017-2055-0

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  • DOI: https://doi.org/10.1007/s00706-017-2055-0

Keywords

  • Carbohydrates
  • Enzymes
  • Hydrolysis
  • Oxidation
  • Rhamnosidase