Characterization of a laboratory-constructed miniaturized device for fast CE measurements with contactless conductivity, amperometric, and mass spectrometry detection
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In the recent years, attention for fast separation of all kind of samples in combination with automated systems has increased. On that account we designed and constructed a miniaturized capillary electrophoresis system which can achieve fast separations due to short capillary length and high electric field strength. An integrated and exchangeable autosampler unit which can be loaded with 19 samples at once allowed high-throughput measurements with small sample volumes down to 30 mm3. The design of the device enables the implementation of various detectors. The aim of this work was the analytical characterization of this device in combination with mass spectrometry, amperometric and capacitively coupled contactless conductivity detection. Hydrodynamic and electrokinetic injections were carried out with different injection parameters. Different model systems for each detection technique were used to test the performance of the device, concerning speed of separation, precision, resolution, and sample throughput.
KeywordsCapillary zone electrophoresis Amino acids Mass spectrometry Amperometric detection Contactless conductivity detection