Monatshefte für Chemie - Chemical Monthly

, Volume 148, Issue 9, pp 1661–1665 | Cite as

Characterization of a laboratory-constructed miniaturized device for fast CE measurements with contactless conductivity, amperometric, and mass spectrometry detection

  • Andreas Schmidberger
  • Sebastian Piendl
  • Jonas Josef Peter Mark
  • Frank-Michael MatysikEmail author
Original Paper


In the recent years, attention for fast separation of all kind of samples in combination with automated systems has increased. On that account we designed and constructed a miniaturized capillary electrophoresis system which can achieve fast separations due to short capillary length and high electric field strength. An integrated and exchangeable autosampler unit which can be loaded with 19 samples at once allowed high-throughput measurements with small sample volumes down to 30 mm3. The design of the device enables the implementation of various detectors. The aim of this work was the analytical characterization of this device in combination with mass spectrometry, amperometric and capacitively coupled contactless conductivity detection. Hydrodynamic and electrokinetic injections were carried out with different injection parameters. Different model systems for each detection technique were used to test the performance of the device, concerning speed of separation, precision, resolution, and sample throughput.

Graphical abstract


Capillary zone electrophoresis Amino acids Mass spectrometry Amperometric detection Contactless conductivity detection 


  1. 1.
    Jorgenson JW, Lukacs KD (1981) Anal Chem 53:1298CrossRefGoogle Scholar
  2. 2.
    Matysik FM (2010) Anal Bioanal Chem 397:961CrossRefGoogle Scholar
  3. 3.
    Ryvolvá M, Macka M (2010) Trends Anal Chem 29:339CrossRefGoogle Scholar
  4. 4.
    Sung WC, Makamba H, Chen SH (2005) Electrophoresis 26:1783CrossRefGoogle Scholar
  5. 5.
    Van Schepdael A (2016) Separations 3:12CrossRefGoogle Scholar
  6. 6.
    Koenka IJ, Küng N, Kuban P, Chwalek T, Furrer G, Wehrli B, Müller B, Hauser PC (2016) Electrophoresis 37:2368CrossRefGoogle Scholar
  7. 7.
    Bonvin G, Rudaz S, Schappler J (2012) J Chromatogr 1262:17CrossRefGoogle Scholar
  8. 8.
    Kuban P, Nguyen HTA, Macka M, Haddad PR, Hauser PC (2007) Electroanalysis 19:2059CrossRefGoogle Scholar
  9. 9.
    Francisco KJM, do Lago CL (2009) Electrophoresis 30:3458CrossRefGoogle Scholar
  10. 10.
    Sloss S, Ewing AG (1993) Anal Chem 65:577CrossRefGoogle Scholar
  11. 11.
    Grundmann M, Matysik FM (2012) Anal Bioanal Chem 404:1713CrossRefGoogle Scholar
  12. 12.
    Vochyánová B, Opekar F, Tůma P (2012) Anal Bioanal Chem 404:1549CrossRefGoogle Scholar
  13. 13.
    Mark JJP, Piccinelli P, Matysik FM (2014) Anal Bioanal Chem 406:6069CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  • Andreas Schmidberger
    • 1
  • Sebastian Piendl
    • 1
  • Jonas Josef Peter Mark
    • 2
  • Frank-Michael Matysik
    • 1
    Email author
  1. 1.Faculty of Chemistry and Pharmacy, Institute for Analytical Chemistry, Chemo- and BiosensorsUniversity of RegensburgRegensburgGermany
  2. 2.MARAWERegensburgGermany

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