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Monitoring the DNA by ruthenium complexes of heterocyclic N,S-donor ligands and evaluation of biological activities

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Abstract

Neutral N,S-donor bidentate ligands have been synthesized and characterized by NMR and IR spectroscopic techniques. The ligands have been used to synthesized ruthenium(II) complexes ([Ru(L1–L6)PPh3)2Cl2]) and ruthenium(III) complexes ([Ru(L1–L6)PPh3)Cl3]). Synthesized complexes have been characterized using elemental analysis, UV–Vis spectroscopy, magnetic measurements, LC–MS, and IR spectroscopy. Broth dilution technique was used to investigate antibacterial activity against two Gram-positive and three Gram-negative microorganisms and results show that all complexes are more potent than the respective ligands. UV–Vis absorption titration and viscosity measurements have been carried out to investigate the binding mode and binding strength of complexes with herring sperm DNA. The quantitative binding strength (K b) of the complexes was found in the range of 0.521 × 105–2.94 × 105 M−1 and indicate intercalative mode of binding. Gel electrophoresis study was carried out to study the cleavage of pUC19 supercoiled DNA. DNA nuclease activity data for the complexes are found higher than respective ligands and metal salt. Brine shrimp bioassay was carried out to perform cytotoxicity study. IC50 values of the complexes were found in the range of 5.69 ± 1.06–14.62 ± 0.87 μg cm−3.

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References

  1. Meggers E (2011) Angew Chem Int Ed 50:2442

    Article  CAS  Google Scholar 

  2. Meggers E (2009) Chem Commun 7:1001

    Article  Google Scholar 

  3. Rosenberg B, Vancamp L, Trosko J, Mansour V (1969) Nature 222:385

    Article  CAS  Google Scholar 

  4. Wheate N, Walker S, Craig G, Oun R (2010) Dalton Trans 39:8113

    Article  CAS  Google Scholar 

  5. Gallerani E, Bauer J, Hess D, Boehm S, Droege C, Jeckelmann S, Miani M, Herrmann R, Marsoni S, Sperka S, Sessa C (2011) Acta Oncol 50:1105

    Article  CAS  Google Scholar 

  6. Todd R, Lippard S (2009) Metallomics 1:280

    Article  CAS  Google Scholar 

  7. Galanski M, Jakupec M, Keppler B (2005) Curr Med Chem 12:2075

    Article  CAS  Google Scholar 

  8. Ott I, Gust R (2007) Arch Pharm Chem Life Sci 340:117

    Article  CAS  Google Scholar 

  9. Clarke M, Zhu F, Frasca D (1999) Chem Rev 99:2511

    Article  CAS  Google Scholar 

  10. Novakova O, Kasparkova J, Vrana O, Van Vliet P, Reedijk J, Brabec V (1995) Biochemistry 34:12369

    Article  CAS  Google Scholar 

  11. Velders A, Kooijman H, Spek A, Haasnoot J, De Vos D, Reedijk J (2000) Inorg Chem 39:2966

    Article  CAS  Google Scholar 

  12. Morris RE, Aird RE, del Socorro Murdoch P, Chen H, Cummings J, Hughes N, Parsons S, Parkin A, Boyd G, Jodrell D, Sadler P (2001) J Med Chem 44:3616

    Article  CAS  Google Scholar 

  13. Alessio E, Mestroni G, Bergamo A, Sava G (2004) Curr Top Med Chem 4:1525

    Article  CAS  Google Scholar 

  14. Hartinger C, Zorbas-Seifried S, Jakupec M, Zorbas H, Keppler B (2006) J Inorg Biochem 100:891

    Article  CAS  Google Scholar 

  15. Patel M, Patel C, Joshi H, Thakor K (2014) Spectrochim Acta A 127:261

    Article  CAS  Google Scholar 

  16. Pyle A, Rehmann J, Meshoyrer R, Kumar C, Turro N, Barton J (1989) J Am Chem Soc 111:3053

    Google Scholar 

  17. Chao H, Mei W, Huang Q, Ji L (2002) J Inorg Biochem 92:165

    Article  CAS  Google Scholar 

  18. Sphillane C, Smith J, Buck D, Collins JG, Keene FR (2007) Dalton Trans 45:5290

    Article  Google Scholar 

  19. Friedman A, Chambron J, Sauvage J, Turro N, Barton J (1990) J Am Chem Soc 112:4960

    Article  CAS  Google Scholar 

  20. Xian-Lan H, Hong L, Cui-Hong P (2011) J Mol Struc 990:197

    Article  Google Scholar 

  21. Li-Feng T, Hui C, Kang-Cheng Z, Jun-Jie F, Fang W, Yang-Fan Z, Liang-Nian J (2007) Polyhedron 26:5458

    Article  Google Scholar 

  22. Krohnke F (1976) Synthesis 1976:1

    Article  Google Scholar 

  23. Garza-Ortiz A, Maheswari PU, Siegler M, Spek A, Reedijk J (2013) New J Chem 37:3450

    Article  CAS  Google Scholar 

  24. Ruminski R, Kahrilas G, Schauer B (2015) Inorg Chim Acta 430:184

    Article  CAS  Google Scholar 

  25. Barton J, Dannenberg J, Raphael A (1984) J Am Chem Soc 106:2172

    Article  CAS  Google Scholar 

  26. Smith S, Neyhart G, Karlsbeck W, Thorp H (1994) New J Chem 18:397

    CAS  Google Scholar 

  27. Carter M, Rodrigues M, Bard A (1989) J Am Chem Soc 111:8901

    Article  CAS  Google Scholar 

  28. Leng F, Priebe W, Chaires J (1998) Biochemistry 37:1743

    Article  CAS  Google Scholar 

  29. Meyer B, Ferrigni N, Putnam J, Jacobsen L, Nichols D, McLaughlin J (1982) Planta Med 45:31

    Article  CAS  Google Scholar 

  30. Hertzberg R, Dervan P (1982) J Am Chem Soc 104:313

    Article  CAS  Google Scholar 

  31. Sigman D, Graham D, Marshall L, Reich K (1980) J Am Chem Soc 102:5419

    Article  Google Scholar 

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Acknowledgments

We are thankful to the Head of chemistry department and UGC RFSMS Scheme for providing financial support.

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Authors and Affiliations

  1. Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar, 388 120, Gujarat, India

    Parag S. Karia, Pankajkumar A. Vekariya, Anshul P. Patidar & Mohan N. Patel

  2. Department of Bioscience, Sardar Patel University, Vallabh Vidyanagar, 388 120, Gujarat, India

    Ravi R. Patel

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  1. Parag S. Karia
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  2. Pankajkumar A. Vekariya
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  3. Anshul P. Patidar
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  5. Mohan N. Patel
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Correspondence to Mohan N. Patel.

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Karia, P.S., Vekariya, P.A., Patidar, A.P. et al. Monitoring the DNA by ruthenium complexes of heterocyclic N,S-donor ligands and evaluation of biological activities. Monatsh Chem 147, 1903–1914 (2016). https://doi.org/10.1007/s00706-016-1708-8

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  • DOI: https://doi.org/10.1007/s00706-016-1708-8

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