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Verification of antibody labelling efficiency as an important step in ELISA/QLISA development

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Abstract

A simple and effective method for validation of antibody labelling procedure as well as for optimal conjugate dilution assessment based on electrochemical detection is presented. Enzyme alkaline phosphatase and core-shell CdSe/ZnS quantum dots are used as sensitive tag exploitable as detection tool of antibody-antigen complex in magnetic microbead-based immunoassays. Square-wave voltammetry for detection of the electroactive product that is induced by conversion of appropriate substrate by alkaline phosphatase and square-wave anodic-stripping voltammetry for signal of Cd in case of quantum dots, respectively, was elected. Anti-Apo E IgG antibodies were selected as model molecules for method optimizations.

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Acknowledgments

Support of the Czech Science Foundation Grant No. 15-16549S and University of Pardubice, Faculty of Chemical Technology (Project No. SGFChT07/2015) is gratefully acknowledged.

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    Authors and Affiliations

    1. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic

      Michaela Čadková, Veronika Dvořáková, Zuzana Bílková & Lucie Korecká

    2. Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic

      Radovan Metelka

    Authors
    1. Michaela Čadková
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    2. Veronika Dvořáková
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    3. Radovan Metelka
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    4. Zuzana Bílková
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    5. Lucie Korecká
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    Corresponding author

    Correspondence to Lucie Korecká.

    Additional information

    M. Čadková and V. Dvořáková contributed equally to this work.

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    Čadková, M., Dvořáková, V., Metelka, R. et al. Verification of antibody labelling efficiency as an important step in ELISA/QLISA development. Monatsh Chem 147, 69–73 (2016). https://doi.org/10.1007/s00706-015-1598-1

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    • DOI: https://doi.org/10.1007/s00706-015-1598-1

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