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Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

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Abstract

Differential pulse voltammetry, cyclic voltammetry, and chronocoulometry at a hanging mercury drop electrode were used for the study of the interaction of methyl violet 2B (MV) with double-stranded DNA in 0.1 mol dm−3 acetate buffer pH 4.0. The mechanism of the electrochemical reduction of MV was also investigated, using cyclic voltammetry at a hanging mercury drop electrode. UV–Vis spectrophotometry was used for the investigation of the interaction as well. The kinetic parameters of the reduction of MV and the DNA–MV complex and the thermodynamic parameters of the formed DNA–MV complex were calculated. Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in acetate buffer was constructed to be used for the indirect determination of DNA based on the decreasing peak current of MV with increasing concentration of DNA in the measured solution.

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Acknowledgments

This research was carried out within the framework of the Specific University Research (SVV). Financial support from the Grant Agency of the Czech Republic (Project P206/12/G151) is gratefully acknowledged.

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Authors and Affiliations

  1. Charles University in Prague, Faculty of Science, University Research Centre UNCE “Supramolecular Chemistry”, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, CZ-128 43, Prague 2, Czech Republic

    Eva Horakova, Vlastimil Vyskocil & Jiri Barek

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  1. Eva Horakova
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  2. Vlastimil Vyskocil
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  3. Jiri Barek
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Correspondence to Eva Horakova.

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Horakova, E., Vyskocil, V. & Barek, J. Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions. Monatsh Chem 147, 119–126 (2016). https://doi.org/10.1007/s00706-015-1590-9

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  • DOI: https://doi.org/10.1007/s00706-015-1590-9

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