Abstract
In this study the interaction of sulfamethazine with DNA was investigated by photochemically induced fluorescence, UV–Vis spectroscopy, and voltammetry on the glassy carbon electrode modified with DNA-multiwalled carbon nanotubes composite (DNA/MWCNT/GCE). Photochemically induced fluorescence was based on UV irradiation of sulfamethazine in the presence of sodium sulfite as sensitizing reagent for a fixed time. The photochemically induced fluorescence emitted by sulfamethazine at 348 nm could be quenched in B-R buffer solution (pH 7.0, 0.04 mol dm−3) when DNA was added, which indicating the interaction of sulfamethazine with DNA. The mechanism of fluorescence quenching was a static quenching according to the Stern–Volmer plot. Furthermore, interaction of sulfamethazine with DNA on DNA/MWCNT/GCE, showing a decrease of peak current of the sulfamethazine oxidation without significant potential shift. The UV spectroscopic data confirmed the intercalative interaction by hypochromism and bathochromic shift of 242 nm absorbance band. The binding constants of the interaction of sulfamethazine with DNA were obtained by all three methods in the range 1.21 × 103–2.2 × 103 dm3 mol−1. Finally, photochemically induced fluorescence was successfully applied for determination of DNA with satisfactory linearity ranges (0.7–8.1 and 13.1–65.0 mg dm−3), admissible limit of detection (0.43 mg dm−3), and good repeatability (0.29 %).
Graphical abstract
Similar content being viewed by others
References
Mandong G, Yanging L, Hongxia G, Xiaoqin W, Lifang F (2007) Bioelectrochemistry 70:245
Baylin SB, Ohm JE (2006) Nat Rev Cancer 6:107
Wang Q, Wu Q, Wang J, Chen D, Fan P, Wang B (2014) Spectrochim Acta A 117:754
Raman N, Sobha S, Selvaganapathy M (2012) Monatsh Chem 143:1487
Hu Y, Lai G, Shen Y, Li Y (2004) Monatsh Chem 135:1167
Patel MN, Patidar AP (2014) Monatsh Chem 145:369
De Zayas-Blanco F, Garcia-Falcon MS, Simal-Gandara J (2004) J Food Control 15:375
Zhang H, Deng X, Miki D, Cutler S, La H, Hou Y-J, Oh J, Zhua J-K (2012) Plant Cell 24:1230
Poirier LA, Doerge DR, Gaylor DW, Miller MA, Lorentzen RJ, Casciano DA, Kadlubar FF, Schwetz BA (1999) Regul Toxicol Pharm 30:217
Sanchez-Pena M, Salinas F, Mahedero MC, Aaron JJ (1994) Talanta 41:233
He Q, Chen H, Cao X (1999) Microchem J 61:125
Mashhadizadeh MH, Pourtaghavi Talemi R (2014) Spectrochim Acta A 132:403
Yu X, Chai Y, Jiang J, Cui H (2012) J Photochem Photobiol A 241:45
Fotouhi L, Atoofi Z, Heravi MM (2013) Talanta 103:194
Fotouhi L, Tabatabaee R (2014) Spectrochim Acta A 124:152
Fotouhi L, Hashkavayi AB, Heravi MM (2013) Int J Biol Macromol 53:101
Lakowicz JR (1986) Principles of fluorescence spectroscopy, 2nd edn. Plenum Press, New York
Feng XZ, Lin Z, Yang LJ, Wang Ch, Bai ChI (1998) Talanta 47:1223
Voorhies JD, Adams RN (1958) Anal Chem 30:346
Astrid MV, Maria E, Carrera B, Dietrich VB, Carls BF, Malcolm RS (1984) Anal Chim Acta 159:119
Sharp M, Petersson M, Edstrom K (1979) J Electroanal Chem 95:123
Laviron E (1979) J Electroanal Chem 101:19
Ju H, Ye Y, Zhu Y (2005) Electrochim Acta 50:1361
Ni Y, Lin D, Kokot S (2005) Talanta 65:1295
Nie MY, Wang Y, Li HL (1997) Pol J Chem 71:816
Nakashima N, Okuzono S, Marakami H, Nakai T, Yoshikawa K (2003) Chem Lett 32:456
Danial S, Rao TP, Rao KS, Rani SU, Naidu GRK, Lee HY, Kawai T (2007) Sens Actuators B Chem 122:672
Li YQ, Guoa YJ, Li XF, Pan JH (2007) Talanta 71:123
Wei W, Hao Q, Zhou Q, Wu J, Lu L, Wang X, Yang X (2008) Electrochim Acta 53:7338
Xu B, Jiao K, Ren Y, Sun W (2007) J Chin Chem Soc 54:917
Chen J, Zhang J, Zhuang Q, Chen J, Lin X (2007) Electroanal 19:1765
Feng Q, Li NQ, Jiang YY (1997) Anal Chim Acta 344:97
Acknowledgments
The authors gratefully acknowledge partial financial support from the Research Council of Alzahra University.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fotouhi, L., Zabeti, M. Photochemically induced fluorimetry, UV–Vis spectroscopy, and voltammetry on the DNA/MWCNT/GCE to investigate the interaction of sulfamethazine with DNA: determination of DNA. Monatsh Chem 147, 837–844 (2016). https://doi.org/10.1007/s00706-015-1518-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00706-015-1518-4