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Synthesis of polyaniline using electrochemical polymerization and application in a sensitive DNA biosensor with [Ru(bpy)3]2+ functionalized nanoporous gold composite as label

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Abstract

We describe here a sensitive sandwich-type electrochemiluminescence DNA biosensor based on a polyaniline–nanogold (PANI–AuNPs) modified platform with tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate ([Ru(bpy)3]2+)–nanoporous gold (Ru–NPG) composite as a label. The PANI was obtained by electropolymerization of aniline on the surface of indium–tin oxide (ITO) glass and gold nanoparticles (AuNPs) were fabricated by the reaction of PANI. The fabricated AuNPs were further grown to a larger size in HAuCl4 solution and the capture DNA was fastened onto the ITO glass on the basis of the interaction with AuNPs. NPG was prepared by a selective dissolution of silver from silver–gold alloy in nitric acid, which provided free-standing noble metal membranes with controllable three-dimensional porosity to immobilize more [Ru(bpy)3]2+. On the basis of a sandwich-type hybridization reaction, the Ru–PANI labeled reporter DNA was captured by the target DNA. The as-proposed DNA biosensor enables target DNA concentrations to be determined in the 0.05–1,000 fM concentration range, with a detection limit of 0.01 fM.

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Acknowledgments

This work was financially supported by the Natural Science Research Foundation of China (51273084, 51003039 31171700), Technology Development Plan of Shandong Province, China (Grant No. 2012GGB01181).

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Authors and Affiliations

  1. Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, China

    Xiu Wang, Weiyan Liu, Chen Li, Chengchao Chu, Shaowei Wang, Mei Yan, Jinghua Yu & Jiadong Huang

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  1. Xiu Wang
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  2. Weiyan Liu
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  3. Chen Li
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  4. Chengchao Chu
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  5. Shaowei Wang
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  6. Mei Yan
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  7. Jinghua Yu
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  8. Jiadong Huang
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Correspondence to Jiadong Huang.

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Wang, X., Liu, W., Li, C. et al. Synthesis of polyaniline using electrochemical polymerization and application in a sensitive DNA biosensor with [Ru(bpy)3]2+ functionalized nanoporous gold composite as label. Monatsh Chem 144, 1759–1765 (2013). https://doi.org/10.1007/s00706-013-1073-9

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  • DOI: https://doi.org/10.1007/s00706-013-1073-9

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