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Magnetic nanoparticle-based electrochemiluminescent immunosensor for detection of carcinoembryonic antigen based on silica nanosphere@gold nanoparticles-Ru as labels

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Abstract

In this article, we propose a novel electrochemiluminescence immunosensor for the detection of carcinoembryonic antigen on a portable indium tin oxide device. The gold nanoparticle-coated silica nanosphere aggregated with Ru(bpy) 2+3 was used as an excellent label with the signal amplification technique. The amino-functionalized Fe3O4 magnetic nanoparticles were treated with 3-(2-aminoethylamino)propyltrimethoxysilane. The primary monoclonal antibody to α-carcinoembryonic antigen was immobilized onto the surface of Fe3O4 by glutaraldehyde as coupling agent. Under optimal conditions, the concentrations of carcinoembryonic antigen were obtained in the linear range of 0.001–10 ng cm−3 with a detection limit of 0.3 pg cm−3. The resulting immunosensor possessed high sensitivity, good stability, reproducibility, satisfied regeneration and selectivity, and could be used in other biological assays.

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Acknowledgments

This work was financially supported by the Natural Science Research Foundation of China (21277058, 21175058), Natural Science Foundation of Shandong Province, China (ZR2012BZ002), and Technology Development Plan of Shandong Province, China (Grant No. 2011GGB01153).

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Authors and Affiliations

  1. Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Meng Li, Yan Zhang, Meng Zhang, Mei Yan, Shenguang Ge & Jinghua Yu

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  1. Meng Li
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  2. Yan Zhang
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  3. Meng Zhang
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  4. Mei Yan
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  5. Shenguang Ge
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Correspondence to Jinghua Yu.

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Li, M., Zhang, Y., Zhang, M. et al. Magnetic nanoparticle-based electrochemiluminescent immunosensor for detection of carcinoembryonic antigen based on silica nanosphere@gold nanoparticles-Ru as labels. Monatsh Chem 145, 113–120 (2014). https://doi.org/10.1007/s00706-013-0983-x

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  • DOI: https://doi.org/10.1007/s00706-013-0983-x

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