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Analytical methods for detection of small amounts of amino groups on solid surfaces: a survey

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Abstract

Various analytical methods have been performed or adapted for the detection of small amounts of amino groups on solid surfaces. As well as the quantification of available amino groups, area-wide coatings should be detectable for applications such as biocompatible coatings of medically relevant materials. In this regard, photometric and fluorometric methods have been used and an enzymatic assay developed, all of which are compared herein.

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Acknowledgments

Thanks to Prof. Irene Lichtscheidl for allocation of the fluorescence microscope.

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Authors and Affiliations

  1. Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, 1030, Vienna, Austria

    Helmut H. Hinterwirth, Martina Strobl & Haifa Al-Dubai

Authors
  1. Helmut H. Hinterwirth
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  2. Martina Strobl
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  3. Haifa Al-Dubai
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Corresponding author

Correspondence to Haifa Al-Dubai.

Additional information

H. H. Hinterwirth and M. Strobl contributed equally to this work.

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Hinterwirth, H.H., Strobl, M. & Al-Dubai, H. Analytical methods for detection of small amounts of amino groups on solid surfaces: a survey. Monatsh Chem 141, 291–299 (2010). https://doi.org/10.1007/s00706-010-0251-2

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  • DOI: https://doi.org/10.1007/s00706-010-0251-2

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