Skip to main content
SpringerLink
Log in

Sol–gel encapsulation of acid phosphatase in the presence of the ionic liquid [BMIM][BF4]

  • Original Paper
  • Published:
Monatshefte für Chemie - Chemical Monthly Aims and scope Submit manuscript

Abstract

The effect of the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]) on acid phosphatase (APase) from wheat germ in solution and in the sol–gel-encapsulated form was investigated to explore new methods of enzyme preparation with improved catalytic performance. APase was encapsulated in hydrogel beads made from tetramethyl orthosilicate. Compared with free APase, 20–28% enzymatic activity was retained in over nine catalytic cycles. Sol–gel encapsulation improved the thermal stability of APase. Heat shock exposure at 60 °C for 1 h resulted in activity decreasing by a factor of three, only, for sol–gel-encapsulated APase, in contrast with a factor of nine decrease for free APase. Addition of 10% v/v [BMIM][BF4] did result in a slight decrease of enzymatic activity for free and sol–gel-encapsulated APase, but resulted in a remarkable increase in alkaline pH tolerance of sol–gel-encapsulated APase.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Gill I (2001) Chem Mater 13:3404

    Article  CAS  Google Scholar 

  2. Wei Y, Xu JG, Feng QW, Lin MD, Dong H, Zhang WJ, Wang C (2001) J Nanosci Nanotechnol 1:83

    Article  CAS  Google Scholar 

  3. O’Neill H, Angley CV, Hemery I, Evans BR, Dai S, Woodward J (2002) Biotechnol Lett 24:783

    Article  Google Scholar 

  4. Frenkel-Mullerad H, Avnir D (2005) J Am Chem Soc 127:8077

    Article  CAS  Google Scholar 

  5. Kragl U, Eckstein M, Kaftzik N (2002) Curr Opin Biotech 13:565

    Article  CAS  Google Scholar 

  6. Karout A, Pierre AC (2007) J Non-Cryst Solids 353:2900

    Article  CAS  Google Scholar 

  7. Liu Y, Wang MJ, Li J, Li ZY, He P, Liu HT, Li JH (2005) Chem Commun 1778

  8. Lee SH, Doan TTN, Ha SH, Koo YM (2007) J Mol Catal B Enzym 45:57

    Article  CAS  Google Scholar 

  9. Joyce BK, Grisolia S (1960) J Biol Chem 235:2278

    CAS  Google Scholar 

  10. He ZQ, Griffin TS, Honeycutt CW (2004) J Environ Qual 33:367

    Article  CAS  Google Scholar 

  11. Machado MF, Saraiva JM (2005) Biotechnol Lett 27:1233

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The principal investigator (Monika Sommerhalter) received a Faculty Research Grant and an Interdisciplinary Sieber-Tombari Research Award from California State University, East Bay. The first author (Suhasini Kulkarni) received a graduate student research grant from California State University, East Bay. The second author (Elizabeth Chin) gratefully acknowledges financial support from the CSU-LSAMP program which is supported by the National Science Foundation under Grant No. HRD-0331537. CD measurements were performed in the laboratory of Professor Daryl Eggers at San Jose State University. We thank Dr Eggers for his kind hospitality and support during the measurements.

Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, California State University, East Bay, Hayward, CA, USA

    Suhasini Kulkarni, Elizabeth Chin, Vu Tran, Maggie K.-M. Ho, Chieu Phan & Monika Sommerhalter

Authors
  1. Suhasini Kulkarni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elizabeth Chin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vu Tran
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maggie K.-M. Ho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chieu Phan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Monika Sommerhalter
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Monika Sommerhalter.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kulkarni, S., Chin, E., Tran, V. et al. Sol–gel encapsulation of acid phosphatase in the presence of the ionic liquid [BMIM][BF4]. Monatsh Chem 141, 119–123 (2010). https://doi.org/10.1007/s00706-009-0230-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00706-009-0230-7

Keywords

Search

Navigation