Summary.
A novel tospovirus serologically distinct from all established tospo- virus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides. The ambisense RNA segment encoded a nonstructural protein (NSs) of 469 amino acids, with a predicted Mr of 53.2 kDa, and a nucleoprotein (N) of 279 amino acids and a Mr of 31.0 kDa, so far the largest N protein known for any tospovirus species. N protein sequence comparisons revealed closest relationship to the species Watermelon bud necrosis virus (58% identity), Watermelon silver mottle virus and Peanut bud necrosis virus (57%) and a distant relationship to Peanut yellow spot virus (23%) and Peanut chlorotic fanspot virus (22%).
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Received March 27, 2000 Accepted July 27, 2000
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Cortez, I., Saaijer, J., Wongjkaew, K. et al. Identification and characterization of a novel tospovirus species using a new RT-PCR approach. Arch. Virol. 146, 265–278 (2001). https://doi.org/10.1007/s007050170174
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DOI: https://doi.org/10.1007/s007050170174