Summary
To understand the topology and mechanism of poliovirus uncoating, the question of whether intact virions can be endocytosed by the host cell was studied by a combination of various techniques. In order to prevent alteration of the virus to subviral particles, Hela cells were infected at 26 °C. At this temperature the majority of cell-associated virions remained at the plasma membrane, whereas a smaller amount accumulated in vesicles having the same mobility (upon free-flow electrophoresis) and migration behaviour on Nycodenz density gradients as early and late endosomes. Co-localization of native poliovirions with endosomal markers was verified by peroxidase-induced diaminobenzidine density-shift of endosomal vesicles. Internalization of poliovirions into endosomes makes it likely, but does not prove that viral RNA can be released into the cytoplasm from the vesicular compartment.
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Received October 16, 1997 Accepted February 5, 1998
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Kronenberger, P., Schober, D., Prchla, E. et al. Uptake of poliovirus into the endosomal system of HeLa cells. Arch. Virol. 143, 1417–1424 (1998). https://doi.org/10.1007/s007050050385
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DOI: https://doi.org/10.1007/s007050050385