Summary
We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a monomeric and possibly a trimeric structure is detected in cell supernatant, irrespective of the pH of the medium. Evidence is presented that the aggregated fraction is generated out of monomeric HA0s molecules due to a low intracellular pH encountered during secretion.
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Accepted October 9,1997 Received July 22, 1997
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Vanlandschoot, P., Beirnaert, E., Grooten, J. et al. pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin. Arch. Virol. 143, 227–239 (1998). https://doi.org/10.1007/s007050050282
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DOI: https://doi.org/10.1007/s007050050282