Barley yellow dwarf virus-PAV RNA does not have a VPg

Summary

The genus Luteovirus is divided into two subgroups. It has already been shown that a genome-linked protein (VPg) is covalently attached to the 5′-end of the genomic RNA of subgroup II luteoviruses, but whether a VPg is present or absent on the genomic RNA of subgroup I luteoviruses is unknown. In the present work, a simple and sensitive method was developed for the detection of the VPg on virion genomic RNA. This method was used to show that a VPg is not attached to the 5′-end of the RNA genome of barley yellow dwarf virus-PAV which is a member of subgroup I luteoviruses.