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Genome analysis of gyroviruses identified in waterfowl in Arizona (USA)

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Abstract

Gyroviruses are small single-stranded DNA (ssDNA) viruses that are largely associated with birds. Chicken anemia virus is the most extensively studied gyrovirus due to its disease impact on the poultry industry. However, we know much less about gyroviruses infecting other avian species. To investigate gyroviruses infecting waterfowl, we determined six complete genome sequences that fall into three gyrovirus groups, referred to as waterfowl gyrovirus 1 (n = 3), 2 (n = 2), and 3 (n = 1), in organs from hunter-harvested waterfowl from Arizona (USA). The waterfowl gyrovirus 1 variants were identified in multiple organs of a single American wigeon and represent a tentative new species. The waterfowl gyrovirus 2 variants were identified in the livers of two American wigeons and share >70% VP1 nucleotide sequence identity with gyrovirus 9, previously identified in the spleen of a Brazilian Pekin duck (MT318123) and a human fecal sample (KP742975). Waterfowl gyrovirus 3 was identified in a northern pintail spleen sample, and it shares >73% VP1 nucleotide sequence identity with two gyrovirus 13 sequences previously identified in Brazilian Pekin duck spleens (MT318125 and MT318127). These gyroviruses are the first to be identified in waterfowl in North America, as well as in American wigeons and northern pintails.

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Fig. 1
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Data availability

The sequences described in this study have been deposited in the GenBank database under accession numbers OR924327-OR924332.

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Acknowledgements

We would like to sincerely thank Charles Wells, Christopher Klaussen, Colin Shepherd, and Nevin Waters for their indispensable assistance in procuring hunter-harvested specimens.

Funding

The authors have not disclosed any funding.

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Authors and Affiliations

  1. School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA

    Shawnpreet Sahnan, Diego Olivo, Rohan A. Bandoo, Danny Jackson, Michael C. Lund, Hannah McGraw, Melanie Regney, Victor Aguiar de Souza Penha, Julia Neil, Dean Drake, Kevin McGraw & Arvind Varsani

  2. The Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University, Tempe, AZ, 85287, USA

    Shawnpreet Sahnan, Diego Olivo, Joy M. Custer, Rohan A. Bandoo, Michael C. Lund, Melanie Regney, Julia Neil, Arvind Varsani & Simona Kraberger

  3. Ecology & Evolutionary Biology, University of Arizona, Tucson, AZ, 85719, USA

    Danny Jackson

  4. Organismal and Evolutionary Research Programme, University of Helsinki, Helsinki, Finland

    Victor Aguiar de Souza Penha

  5. Center for Evolution and Medicine, Arizona State University, Tempe, AZ, 85287, USA

    Arvind Varsani

  6. Structural Biology Research Unit, Department of Integrative, Biomedical Sciences, University of Cape Town, Observatory, Cape Town, 7925, South Africa

    Arvind Varsani

Authors
  1. Shawnpreet Sahnan
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  2. Diego Olivo
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  3. Joy M. Custer
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  11. Dean Drake
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  13. Arvind Varsani
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Corresponding authors

Correspondence to Arvind Varsani or Simona Kraberger.

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This research did not involve human participants or animals.

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Sahnan, S., Olivo, D., Custer, J.M. et al. Genome analysis of gyroviruses identified in waterfowl in Arizona (USA). Arch Virol 169, 120 (2024). https://doi.org/10.1007/s00705-024-06049-9

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