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Biological characterization and complete genome analysis of the newly isolated Serratia liquefaciens phage vB_SlqS_ZDD2

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Abstract

A novel lytic phage named vB_SlqS_ZDD2 was isolated from hospital sewage using the double-layer agar method with Serratia liquefaciens ATCC 27592 as the host. BLASTn analysis showed that the genome sequence of phage vB_SlqS_ZDD2 did not resemble any other phages in the NCBI database. Phenotype and phylogeny analysis indicated that this phage might be a new member of the class Caudoviricetes. Phage vB_SlqS_ZDD2 has a dsDNA genome of 49,178 bp with 55% GC content and has 73 open reading frames. This phage exhibited strong lytic activity and a wide range of pH (3–12) and temperature tolerance (below 70℃).

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Data availability

The complete genome sequence of phage vB_SlqS_ZDD2 is available in the GenBank database under the accession number OR881364.

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Funding

This project was supported by Shandong Provincial Natural Science Foundation (ZR2022QH132, ZR2016HM34).

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Authors and Affiliations

  1. School of Stomatology, Qingdao University, Qingdao, 266003, China

    Qian Zhao, Chen Chu & Rongtao Yuan

  2. Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, 266001, China

    Linzi Han

  3. Central Laboratories, Qingdao Municipal Hospital Affiliated to University of Health and Rehabilitation Sciences, #5 Donghai Middle Road, Qingdao, 266071, China

    Lili Wang & Likun Zhuang

  4. The Center of Stomaology, Qingdao Municipal Hospital, Affiliated to University of Health and Rehabilitation Sciences, #5 Donghai Middle Road, Qingdao, 266071, China

    Rongtao Yuan

Authors
  1. Qian Zhao
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  2. Linzi Han
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  3. Chen Chu
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  4. Lili Wang
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  5. Likun Zhuang
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Correspondence to Rongtao Yuan.

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Communicated by T. K. Frey

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Zhao, Q., Han, L., Chu, C. et al. Biological characterization and complete genome analysis of the newly isolated Serratia liquefaciens phage vB_SlqS_ZDD2. Arch Virol 169, 104 (2024). https://doi.org/10.1007/s00705-024-06026-2

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