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Complete genome sequence of a novel gammapartitivirus from Penicillium oxalicum RCEF7482

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Abstract

Penicillium oxalicum, an important biocontrol fungus in China, has been a subject of extensive study due to its role in combating various pathogenic fungi. Despite the prevalence of mycoviruses with double-stranded (ds) RNA genomes in filamentous fungi, there has been no screening of mycoviruses in P. oxalicum. In this report, we describe the identification and characterization of a novel dsRNA virus isolated from P. oxalicum, designated as "Penicillium oxalicum partitivirus 1" (PoPV1). The genome of PoPV1 consists of two dsRNA segments, dsRNA1 (1,770 bp) and dsRNA2 (1,584 bp), each containing a single open reading frame (ORF): ORF1 and ORF2. Comparative analysis revealed that the RdRp and CP amino acid sequences of PoPV1 share the highest identity (89.18% and 73.97%, respectively) with those of Penicillium aurantiogriseum partitivirus 1 (PaPV1). Motif analysis based on RdRp amino acid sequences places PoPV1 in the genus Gammapartitivirus within the family Partitiviridae, with a distinctive motif VI (R/KV/ILGDD). Phylogenetic analysis further established a close relationship of PoPV1 to PaPV1, forming a unique clade among the gammapartitiviruses. Consequently, we propose that Penicillium oxalicum partitivirus 1 represents a new species in the genus Gammapartitivirus. This is the first report of a dsRNA virus in P. oxalicum.

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Data availability

The datasets generated and/or analysed in the current study are available in the GenBank database.

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Funding

This work was supported by the National Natural Science Foundation of China (grant no. 32172473).

Author information

Author notes

  1. Jing Sun and Ping Wang contributed equally to this study and share first authorship.

Authors and Affiliations

  1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China

    Jing Sun, Ping Wang, Hanwen Lu & Bo Huang

  2. School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China

    Guogen Yang

Authors
  1. Jing Sun
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  2. Ping Wang
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  3. Hanwen Lu
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  4. Guogen Yang
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  5. Bo Huang
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Contributions

BH conceived and designed the study. JS and PW wrote the manuscript, conducted the experiments, and analyzed the data. HL and GY did a part of the experiments. BH edited the manuscript and supervised the project. All authors read and approved the manuscript.

Corresponding author

Correspondence to Bo Huang.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The authors have no conflict of interest.

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Communicated by Massimo Turina

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Sun, J., Wang, P., Lu, H. et al. Complete genome sequence of a novel gammapartitivirus from Penicillium oxalicum RCEF7482. Arch Virol 169, 48 (2024). https://doi.org/10.1007/s00705-024-05987-8

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