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Complete sequence and genome characterization of miscanthus virus M, a new betaflexivirus from Miscanthus sp.

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Abstract

A novel betaflexivirus, tentatively named “miscanthus virus M” (MiVM), was isolated from Miscanthus sp. The complete genome of MiVM is 7,388 nt in length (excluding the poly(A) tail). It contains five open reading frames and has a genome organization similar to those of members of the families Alphaflexiviridae and Betaflexiviridae (subfamily Quinvirinae). The amino acid sequences of both the replicase and coat protein shared less than 45% identity with the corresponding sequences of members of either family. Phylogenetic analysis confirmed that MiVM belongs to the family Betaflexiviridae and subfamily Quinvirinae but it was too distantly related to be included in any currently recognized genus in this family. We therefore propose that miscanthus virus M represents a new species and a new genus in the family Betaflexiviridae.

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Data availability

The viral genomic sequence generated in this work has been deposited in the GenBank database of the National Center for Biotechnology Information (NCBI) under the accession number ON986335.

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Funding

This work was partly funded by an in-house-appropriated project (number 8042-22000-324-000-D).

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Authors and Affiliations

  1. USDA-ARS National Germplasm Resources Laboratory, Beltsville, MD, USA

    Peter Abrahamian, Samuel Grinstead, Gary R. Kinard & Dimitre Mollov

  2. USDA-ARS Molecular Plant Pathology Laboratory, Beltsville, MD, USA

    Samuel Grinstead

  3. USDA-ARS Tropical Agriculture Research Station, Mayaguez, PR, USA

    Ricardo Goenaga

  4. CIRAD, UMR PHIM, Montpellier, France

    Philippe Rott

  5. PHIM Plant Health Institute, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France

    Philippe Rott

  6. USDA-ARS Horticultural Crops Research Unit, Corvallis, OR, 97330, USA

    Dimitre Mollov

Authors
  1. Peter Abrahamian
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  2. Samuel Grinstead
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  3. Gary R. Kinard
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  4. Ricardo Goenaga
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  5. Philippe Rott
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  6. Dimitre Mollov
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Contributions

Conceptualization: DM. Formal analysis: PA, SG. Investigation: PA, SG, RG, PR, DM. Methodology: PA, SG, GRK, RG, PR, DM. Writing – original draft: PA, DM. Writing – review & editing: PA, SG, GRK, RG, PR, DM.

Corresponding authors

Correspondence to Peter Abrahamian or Dimitre Mollov.

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This article does not contain any studies with animals or human participants performed by any of the authors.

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The authors have no relevant financial or non-financial interests to disclose.

Additional information

Communicated by Jesús Navas-Castillo

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Abrahamian, P., Grinstead, S., Kinard, G.R. et al. Complete sequence and genome characterization of miscanthus virus M, a new betaflexivirus from Miscanthus sp.. Arch Virol 169, 27 (2024). https://doi.org/10.1007/s00705-024-05966-z

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  • DOI: https://doi.org/10.1007/s00705-024-05966-z

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