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Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain

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Abstract

Senecavirus A (SVA) is an emerging virus that causes vesicular disease in pigs. Construction of a full-length SVA cDNA clone is crucial for understanding its replication and pathogenesis. Here, we successfully constructed a CMV-promoter-driven infectious cDNA clone of the SVA isolate SVA/GX/CH/2018, which we named rSVA GX01. Sequence comparison between the pSVA GX01 and the parental isolate (SVA/GX/CH/2018) revealed three single-nucleotide differences. Four-week-old piglets were experimentally infected with either the parental virus or the cloned virus. The results showed that the cloned rSVA GX01 displayed weak pathogenicity in 4-week-old pigs compared to the parental virus SVA CH-GX-01-2018. The infectious clone of SVA will serve as a valuable tool for studying the viral replication cycle and for functional analysis of the viral genome.

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Data availability

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.

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Acknowledgements

We are grateful to Dr. Dev Sooranna of Imperial College London for English language editing of the manuscript.

Funding

The Natural Science Foundation of China (31972666) and the Natural Science Foundation of Guangxi Province (No. 2018GXNSFDA281021) funded this research.

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Authors and Affiliations

  1. Laboratory of Animal infectious Diseases and molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, 530005, China

    Hao Wang, Yongfang Mo, Wenbo Liu, Chenxia Niu, Qijie He, Tongwei Ren, Kang Ouyang, Ying Chen, Weijian Huang & Zuzhang Wei

  2. Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530005, China

    Kang Ouyang, Ying Chen, Weijian Huang & Zuzhang Wei

  3. Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, 530005, China

    Kang Ouyang, Ying Chen, Weijian Huang & Zuzhang Wei

  4. Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, Nanning, 530005, China

    Kang Ouyang, Ying Chen, Weijian Huang & Zuzhang Wei

Authors
  1. Hao Wang
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  2. Yongfang Mo
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  3. Wenbo Liu
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  4. Chenxia Niu
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  5. Qijie He
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  6. Tongwei Ren
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  7. Kang Ouyang
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  8. Ying Chen
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  9. Weijian Huang
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  10. Zuzhang Wei
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Contributions

Hao Wang: project administration, writing – original draft. Yongfang Mo: conceptualization. Wenbo Liu: funding acquisition. Chenxia Niu: software. Qijie He: investigation. Tongwei Ren: methodology. Kang Ouyang: resources. Ying Chen: software. Weijian Huang: validation. Zuzhang Wei: visualization, writing – review & editing. All of the authors reviewed the manuscript.

Corresponding author

Correspondence to Zuzhang Wei.

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Conflict of interest

This research was conducted in the absence of any commercial or financial relationships.

Ethics approval

The animal experiments were approved by the Animal Experiment Committee of Guangxi University, with the approval number (GXU2022-266).

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Handling Editor: Ana Cristina Bratanich.

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Wang, H., Mo, Y., Liu, W. et al. Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain. Arch Virol 169, 25 (2024). https://doi.org/10.1007/s00705-023-05951-y

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  • DOI: https://doi.org/10.1007/s00705-023-05951-y

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