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A novel bipartite negative-stranded RNA mycovirus of the order Bunyavirales isolated from the phytopathogenic fungus Fusarium sibiricum

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Abstract

A novel negative-stranded RNA mycovirus was isolated from the phytopathogenic fungus Fusarium sibiricum strain AH32. This virus, tentatively named "Fusarium sibiricum coguvirus 1" (FsCV1), has a bipartite genome consisting of two RNA segments (RNA1 and RNA2). The negative-sense RNA1 is 6711 nt in length, encoding the RNA-dependent RNA polymerase (RdRp, p251) in the viral complementary (vc) strand. The ambisense RNA2 (1204 nt long) encodes two proteins from overlapping genes: the nucleocapsid protein (NP, p38) in the vc strand and a protein of unknown function (UFP, p36) in the viral (v) strand. In contrast to other Bunyavirales members, in FsCV1, the two open reading frames are separated by a long AU-rich intergenic region (IR). Sequence comparisons and phylogenetic analysis based on RdRp and NP sequences demonstrated that FsCV1 is a novel bipartite negative-stranded RNA mycovirus of the genus Coguvirus, family Phenuiviridae, order Bunyavirales.

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Funding

This research was supported by the National Natural Science Foundation of China (32270178) and the National Natural Science Foundation of China (32202381).

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Author notes

  1. Pengfei Li and Pallab Bhattacharjee contributed equally to this work.

Authors and Affiliations

  1. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, 510642, China

    Pengfei Li

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China

    Pallab Bhattacharjee, Shuangchao Wang & Lihua Guo

  3. All-Russian Institute of Plant Protection, Pushkin, St. Petersburg, Russian Federation

    Tatiana Gagkaeva

Authors
  1. Pengfei Li
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  2. Pallab Bhattacharjee
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  3. Tatiana Gagkaeva
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  4. Shuangchao Wang
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  5. Lihua Guo
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Correspondence to Lihua Guo.

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Li, P., Bhattacharjee, P., Gagkaeva, T. et al. A novel bipartite negative-stranded RNA mycovirus of the order Bunyavirales isolated from the phytopathogenic fungus Fusarium sibiricum. Arch Virol 169, 13 (2024). https://doi.org/10.1007/s00705-023-05942-z

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  • DOI: https://doi.org/10.1007/s00705-023-05942-z

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