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Separation of spring viraemia of carp virus from large-volume samples using immunomagnetic beads

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Abstract

A method for separation of spring viraemia of carp virus (SVCV) from large-volume samples using immunomagnetic beads (IMBs) coated with a polyclonal antibody against SVCV was developed. The optimum amount of IMBs was 2 mg in 100 mL. After IMB treatment, the detection limit of SVCV in reverse transcription quantitative PCR (RT-qPCR) was 103 times the 50% tissue culture infectious dose per mL in 100-mL samples. The concentration of viral RNA extracted from SVCV that had been separated using IMBs was 5.18 × 103-fold higher than that of the unseparated SVCV. When fish samples were tested, the concordance rates of the IMBs/RT-qPCR and RT-qPCR were 100% and 67.5%, respectively.

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Fig. 1

Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by the Public Welfare Project of Ningbo in China (2022S169) and the Scientific Research Foundation of the Chinese Academy of Inspection and Quarantine (CAIQ; 2022JK43).

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Authors and Affiliations

  1. Chinese Academy of Inspection and Quarantine, Beijing, 100176, China

    Jing Hongli, Zhang Min, Wang Na & Wu Shaoqiang

  2. Da Chan Bay Customs People’s Republic of China, Shenzhen, 518102, China

    Gao Longying

  3. Ningbo Academy of Oceanology and Fisheries, Ningbo, 315012, China

    Si Liegang, Ge Mingfeng, Xu Shengwei & Wang Jianping

Authors
  1. Jing Hongli
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  2. Zhang Min
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  3. Gao Longying
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  4. Si Liegang
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  5. Wang Na
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  6. Ge Mingfeng
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  7. Xu Shengwei
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  8. Wang Jianping
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  9. Wu Shaoqiang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by JH, ZM, GL, SL, WN, GM, XS, WJ, and WS. The first draft of the manuscript was written by JH, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Wang Jianping or Wu Shaoqiang.

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The authors have no relevant financial or non-financial interests to disclose.

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Handling Editor: Kalpana Agnihotri.

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Hongli, J., Min, Z., Longying, G. et al. Separation of spring viraemia of carp virus from large-volume samples using immunomagnetic beads. Arch Virol 169, 8 (2024). https://doi.org/10.1007/s00705-023-05927-y

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  • DOI: https://doi.org/10.1007/s00705-023-05927-y

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