Abstract
A putative new polerovirus, named “chrysanthemum virus D” (ChVD), was detected in a Chrysanthemum morifolium plant in South Korea. The virus was identified by high-throughput sequencing and confirmed by reverse transcription polymerase chain reaction. The entire ChVD genome is composed of 5,963 nucleotides and contains seven open reading frames (ORF0-5 and ORF3a), which are arranged similarly to those of other poleroviruses. These ORFs encode the putative proteins P0-5 and P3a, respectively. Pairwise amino acid sequence comparisons showed that the ChVD P0-5 and P3a proteins have 30.45–75% sequence identity to the corresponding proteins of other members of the genus Polerovirus. Since one of the species demarcation criteria for the genus Polerovirus is > 10% difference in the amino acid sequence of any gene product, the sequence comparisons indicate that ChVD represents a new species in this genus. Phylogenetic analysis of the P1-P2 and P3 amino acid sequences further indicate that ChVD is a novel polerovirus.
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All data included in this study are available upon request by contact with the corresponding author.
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Acknowledgements
This work was supported by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries) and by the Crop Viruses and Pests Response Industry Technology Development Program (321104-3), funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea. We thank Steven M. Thompson from Edanz (www.edanz.com/ac) for editing a draft of this manuscript.
Funding
This study was funded by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET); through the Crop Viruses and Pests Response Industry Technology Development Program (321104-3), funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea. Also, we thank Steven M. Thompson from Edanz (www.edanz.com/ac) for editing a draft of this manuscript.
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Igori, D., Kim, S.E., Kwon, J.A. et al. Complete nucleotide sequence of chrysanthemum virus D, a polero-like virus. Arch Virol 169, 28 (2024). https://doi.org/10.1007/s00705-023-05924-1
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DOI: https://doi.org/10.1007/s00705-023-05924-1