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Identification and nucleotide sequencing of a novel partitivirus derived from Rhizoctonia solani AG-4 HG III isolate A14

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Abstract

Rhizoctonia solani is a widely disseminated phytopathogen that is found in the soil and is capable of harming many important species of crops. Here, analysis of the R. solani AG-4 HG III strain A14 led to the identification of a novel mycovirus assigned the tentative name "Rhizoctonia solani partitivirus A14" (RsPV-A14), which was subjected to sequencing and associated analyses. This approach revealed that RsPV-A14 harbored two dsRNA segments, 2022 bp (dsRNA1) and 1905 bp (dsRNA2) in length. dsRNA1 was found to contain a single open reading frame (ORF1) that codes for a 622-amino-acid protein with conserved RNA-dependent RNA polymerase (RdRp) motifs, and dsRNA2 was found to contain an ORF (ORF2) that is predicted to code for a 558-amino-acid capsid protein (CP). BLASTp analysis using the putative RdRp of RsPV-A14 showed sequence similarity to partitiviruses, including Rosellinia necatrix partitivirus 7 (50.53% identity), an unclassified partitivirus. Phylogenetic analysis based on RdRp protein sequences suggested that RsPV-A14 is a novel member of the family Partitiviridae.

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Acknowledgements

This research was supported by the National Natural Scientific Foundation of China (32260036, 42067009), the Scientific Research Fund of the Education Department of Yunnan Province (2022Y696), and the Innovative Entrepreneurship Training Program for College Students (202205410226).

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Authors and Affiliations

  1. College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China

    Rui Shi, Bengzha Dong, Qiaodi Wang, Wurong Chen, Zebin Chen, Shuhui Yu, Song Jin & Yanqiong Li

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  1. Rui Shi
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  2. Bengzha Dong
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  3. Qiaodi Wang
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  7. Song Jin
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  8. Yanqiong Li
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Correspondence to Yanqiong Li.

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Shi, R., Dong, B., Wang, Q. et al. Identification and nucleotide sequencing of a novel partitivirus derived from Rhizoctonia solani AG-4 HG III isolate A14. Arch Virol 168, 300 (2023). https://doi.org/10.1007/s00705-023-05923-2

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