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miRNA profiling of primary lamb testicle cells infected with lumpy skin disease virus

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Abstract

In this study, miRNA profiling of cells infected with lumpy skin disease virus (LSDV) was conducted for the first time. When compared to mock-infected cells, LSDV-infected primary lamb testicle (LT) cells showed dysregulation of 64, 85, and 85 miRNAs at 12 hours postinfection (hpi), 48 hpi, and 72 hpi, respectively. While some of these miRNAs were found to be dysregulated at a particular time point following LSDV infection, others were dysregulated at all three time points. Analysis of the differentially expressed miRNA-mRNA interaction networks, Gene Ontology analysis of the predicted targets, and KEGG analysis of highly enriched pathways revealed several cellular factors/pathways involved in protein/ion/enzyme binding, cell differentiation, movement of subcellular components, calcium reabsorption, aldosterone synthesis and secretion, and melanogenesis. Some selected upregulated (oar-mir-379-5p, oar-let-7d, Chr10-18769, Chr2_5162 and oar-miR-493-5p) and downregulated (ChrX-33741, Chr3_8257 and Chr26_32680) miRNAs were further confirmed by quantitative real-time PCR. These findings contribute to our understanding of virus replication, virus-host interactions, and disease pathogenesis, and the differentially expressed miRNAs and their cellular targets may serve as biomarkers as well as novel targets for therapeutic intervention against LSDV.

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Data availability

The datasets generated and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Indian Council of Agricultural Research, New Delhi (grant number IXX16675 to Naveen Kumar).

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Author notes

  1. Sakshi Pandita and Assim Verma have authors contributed equally.

Authors and Affiliations

  1. National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana, 125001, India

    Sakshi Pandita, Assim Verma, Himanshu Kamboj, Ram Kumar, Yogesh Chander, Sanjay Barua, Bhupendra Nath Tripathi & Naveen Kumar

  2. Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125004, India

    Sakshi Pandita

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  1. Sakshi Pandita
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Contributions

Naveen Kumar contributed to the study conception and design. Material preparation was performed by Sakshi Pandita, Assim Verma, Yogesh Chander, and Himanshu Kamboj. Data analysis was performed by Sakshi Pandita, Naveen Kumar, Assim Verma, Yogesh Chander, Himanshu Kamboj, Ram Kumar, Bhupendra Nath Tripathi, and Sanjay Barua. The first draft of the manuscript was written by Naveen Kumar, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Naveen Kumar.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

This study was performed in accordance with the guidelines of the Institutional Biosafety Committee (IBSC) of ICAR-National Research Institute on Equines, Hisar, India. This study did not involve any experiments on animals.

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Pandita, S., Verma, A., Kamboj, H. et al. miRNA profiling of primary lamb testicle cells infected with lumpy skin disease virus. Arch Virol 168, 290 (2023). https://doi.org/10.1007/s00705-023-05917-0

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