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Characterization of a novel endornavirus isolated from the phytopathogenic fungus Rhizoctonia solani

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Abstract

Rhizoctonia solani endornavirus 8 (RsEV8) was isolated from strain XY175 of Rhizoctonia solani AG-1 IA. The full-length genome of RsEV8 is 16,147 nucleotides (nt) in length and contains a single open reading frame that encodes a large polyprotein of 5227 amino acids. The polyprotein contains four conserved domains: viral methyltransferase, putative DEAH box helicase, viral helicase, and RNA-dependent RNA polymerase (RdRp). RsEV8 has a shorter 3'-UTR (58 nt) and a longer 5'-UTR (404 nt). A multiple sequence alignment indicated that the RdRp of RsEV8 possesses eight typical RdRp motifs. According to a BLASTp analysis, RsEV8 shares 39.31% sequence identity with Rhizoctonia cerealis endornavirus-1084-7. Phylogenetic analysis demonstrated that RsEV8 clusters with members of the genus Betaendornavirus.

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Acknowledgments

This work was financially supported by the Fundamental Research Funds for the Central Universities (grant 2021ZKPY005), the National Key Research and Development Program of China (grant 2017YFD0201100), and the Natural Science Foundation of China (grant 31772111).

Funding

This work was supported by Natural Science Foundation of China with grant 31772111.

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Authors and Affiliations

  1. Institute of Vegetable Research, Wuhan Academy of Agricultural Sciences, Wuhan, 430045, Hubei, China

    Yangyi Li, Xingxue Huang, Guolin Zhou, Anhua Ye, Yaohua Deng, Lingfang Shi & Runhua Zhang

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  1. Yangyi Li
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  2. Xingxue Huang
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  3. Guolin Zhou
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  4. Anhua Ye
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  5. Yaohua Deng
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Correspondence to Runhua Zhang.

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Li, Y., Huang, X., Zhou, G. et al. Characterization of a novel endornavirus isolated from the phytopathogenic fungus Rhizoctonia solani. Arch Virol 169, 15 (2024). https://doi.org/10.1007/s00705-023-05915-2

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