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Biological and molecular characterization of a new potexvirus isolated from Adenium obesum

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Abstract

Adenium obesum plants showing virus-like symptoms were collected in several regions of Brazil. Mottling symptoms like those observed in symptomatic plants in the field were reproduced in mechanically inoculated A. obesum plants. This potexvirus was named “desert rose mottle virus” (DRMoV), and its genome sequence was first determined by high-throughput sequencing and then confirmed by Sanger sequencing. The complete genome of DRMoV is 6,781 nt in length, excluding the poly(A) tail, and five ORFs were predicted in order from 5’ to 3’: Rep-TGB1-TGB2-TGB3-CP. Phylogenetic analysis based on Rep amino acid sequences showed different clustering among potexviruses. These data suggest that RDMoV is a new member of the genus Potexvirus, and the binomial name “Potexvirus adenii” is proposed for its species.

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Data availability

The assembled sequence has been submitted to the GenBank database (OR240084).

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Acknowledgments

This research received financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP (grant number 2018/18274-3, and 2021/02179-4). VHB has a postdoctoral scholarship from FAPESP (2020/05563-7).

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Authors and Affiliations

  1. Universidade de São Paulo/Esalq, Depto. de Fitopatologia e Nematologia, Av. Pádua Dias 11, Piracicaba, 13418-900, Brazil

    Vinicius Henrique Bello, Jorge Alberto Marques Rezende & Elliot Watanabe Kitajima

  2. Depto. de Biologia Molecular, Universidade de Brasília, Brasília , Federal District, Brazil

    Tatsuya Nagata

  3. UNESP, Faculdade de Ciências Agronômicas, Botucatu, São Paulo, Brazil

    Julio Massaharu Marubayashi & Renate Krause-Sakate

  4. Depto. de Fitotecnia, Universidade Federal do Piauí, Teresina, PI, Brazil

    Layla de Sá Andrade Medeiros & José Evando Aguiar Beserra Júnior

Authors
  1. Vinicius Henrique Bello
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  2. Tatsuya Nagata
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  3. Julio Massaharu Marubayashi
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  4. Renate Krause-Sakate
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  5. Layla de Sá Andrade Medeiros
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  6. José Evando Aguiar Beserra Júnior
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  7. Jorge Alberto Marques Rezende
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  8. Elliot Watanabe Kitajima
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Contributions

All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Vinicius Henrique Bello, Tatsuya Nagata, Julio Massaharu Marubayashi, Renate Krause-Sakate, Layla de Sá Andrade Medeiros, José Evando Aguiar Beserra Júnior, Jorge Alberto Marques Rezende, and Elliot Watanabe Kitajima. The first draft of the manuscript was written by Vinicius Henrique Bello, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Vinicius Henrique Bello.

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Bello, V.H., Nagata, T., Marubayashi, J.M. et al. Biological and molecular characterization of a new potexvirus isolated from Adenium obesum. Arch Virol 168, 272 (2023). https://doi.org/10.1007/s00705-023-05889-1

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  • DOI: https://doi.org/10.1007/s00705-023-05889-1

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