Abstract
In this study, an NADC34-like strain of porcine reproductive and respiratory syndrome virus (PRRSV), YC-2020, was isolated from a pig farm in Yuncheng, Shanxi Province, China. Phylogenetic and molecular evolutionary analysis showed that the genome sequence of YC-2020 was very similar to those of NADC34-like PRRSV strains in the ORF2-7 region. However, it was more closely related to NADC30-like PRRSV and highly pathogenic (HP) PRRSV in the NSP2 and NSP3-9 coding regions, respectively, suggesting that recombination had occurred between viruses belonging to lineages 1 and 8. Piglets infected with YC-2020 exhibited mild clinical signs, but they had severe histopathological lesions in their lungs. These findings reveal novel genetic and pathogenic features of this isolate.
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The datasets generated and/or analyzed in the current study are available from the corresponding author on reasonable request.
References
Dokland T (2010) The structural biology of PRRSV. Virus Res 154:86–97
Gao J-C, Xiong J-Y, Ye C, Chang X-B, Guo J-C, Jiang C-G, Zhang G-H, Tian Z-J, Cai X-H, Tong G-Z, An T-Q (2017) Genotypic and geographical distribution of porcine reproductive and respiratory syndrome viruses in mainland China in 1996–2016. Vet Microbiol 208:164–172
Guo Z, Chen X-x, Li X, Qiao S, Deng R, Zhang G (2019) Prevalence and genetic characteristics of porcine reproductive and respiratory syndrome virus in central China during 2016–2017: NADC30-like PRRSVs are predominant. MICROBIAL PATHOGENESIS 135
Liu J, Liu C, Xu Y, Yang Y, Li J, Dai A, Huang C, Luo M, Wei C (2022) Molecular Characteristics and Pathogenicity of a Novel Recombinant Porcine Reproductive and Respiratory Syndrome Virus Strain from NADC30-, NADC34-, and JXA1-Like Strains That Emerged in China. Microbiol Spectr 10:e0266722
Song S, Xu H, Zhao J, Leng C, Xiang L, Li C, Fu J, Tang YD, Peng J, Wang Q, Zhao H, An T, Cai X, Zhang H, Tian ZJ (2020) Pathogenicity of NADC34-like PRRSV HLJDZD32-1901 isolated in China. Vet Microbiol 246:108727
Sun Y-F, Liu Y, Yang J, Li W-Z, Yu X-X, Wang S-Y, Li L-A, Yu H (2022) Recombination between NADC34-like and QYYZ-like strain of porcine reproductive and respiratory syndrome virus with high pathogenicity for piglets in China. Transbound Emerg Dis 69:E3202–E3207
Tian K, Yu X, Zhao T, Feng Y, Cao Z, Wang C, Hu Y, Chen X, Hu D, Tian X, Liu D, Zhang S, Deng X, Ding Y, Yang L, Zhang Y, Xiao H, Qiao M, Wang B, Hou L, Wang X, Yang X, Kang L, Sun M, Jin P, Wang S, Kitamura Y, Yan J, Gao GF (2007) Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hallmark. PLOS ONE 2
van Geelen AGM, Anderson TK, Lager KM, Das PB, Otis NJ, Montiel NA, Miller LC, Kulshreshtha V, Buckley AC, Brockmeier SL, Zhang J, Gauger PC, Harmon KM, Faaberg KS (2018) Porcine reproductive and respiratory disease virus: Evolution and recombination yields distinct ORF5 RFLP 1-7-4 viruses with individual pathogenicity. Virology 513:168–179
Wang X, Zhang K, Mo Q, Chen G, Lv J, Huang J, Pang Y, Wang H, Liu W, Huang K, Min X, Ren T, Ouyang K, Chen Y, Huang W, Wei Z (2022) The Emergence and Pathogenesis of Recombinant Viruses Associated with NADC34-like Strains and the Predominant Circulating Strains of Porcine Reproductive and Respiratory Syndrome Virus in Southern China. Viruses 14
Wu Y, Peng O, Xu Q, Li Q, Li W, Lin L, Zhou Q, Cai X, Hu G, He Z, Chen Y, Zhang H (2022) Characterization and Pathogenicity of Two Novel PRRSVs Recombined by NADC30-like and NADC34-like Strains in China. Viruses 14
Xu H, Li C, Li W, Zhao J, Gong B, Sun Q, Tang YD, Xiang L, Leng C, Peng J, Wang Q, Meng F, Yu Y, An T, Cai X, Tian ZJ, Zhang H (2022) Novel characteristics of Chinese NADC34-like PRRSV during 2020–2021. Transbound Emerg Dis 69:e3215–e3224
Zhang HL, Zhang WL, Xiang LR, Leng CL, Tian ZJ, Tang YD, Cai XH (2018) Emergence of novel porcine reproductive and respiratory syndrome viruses (ORF5 RFLP 1-7-4 viruses) in China. Vet Microbiol 222:105–108
Zhao J, Xu L, Xu Z, Deng H, Li F, Sun X, Zhou Y, Zhu L (2022) Emergence and spread of NADC34-like PRRSV in Southwest China. Transbound Emerg Dis 69:E3416–E3424
Zhou L, Wang Z, Ding Y, Ge X, Guo X, Yang H (2015) NADC30-like Strain of Porcine Reproductive and Respiratory Syndrome Virus, China. Emerg Infect Dis 21:2256–2257
Funding
This research was supported by the National Natural Science Foundation of China (No. 31672581).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Yongxin Zhao, Ruiqing Wang, Wei Li, Chenfei Yu, Riteng Zhang, Ruochen Hu, Ruhai Guo, Zengqi Yang, Yefei Zhou, and Xinglong Wang. The first draft of the manuscript was written by Yongxin Zhao, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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All animal-related experiments were performed according to the guidelines of the Ethics Committee at Northwest A&F University.
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Communicated by Diego G. Diel.
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Zhao, Y., Wang, R., Li, W. et al. Genetic and pathogenic characterization of an NADC-34-like isolate of porcine reproductive and respiratory syndrome virus. Arch Virol 168, 205 (2023). https://doi.org/10.1007/s00705-023-05822-6
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DOI: https://doi.org/10.1007/s00705-023-05822-6