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Novel ollusvirus detected in a solitary wild bee species (Osmia taurus) in Japan

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Abstract

Pathogens of wild bees in Japan remain largely unknown. We examined viruses harbored by solitary wild Osmia bees, including Osmia cornifrons and Osmia taurus. Interestingly, the full-length genome of a novel virus (designated as "Osmia-associated bee chuvirus", OABV) was identified in three Osmia taurus bees collected in Fukushima prefecture. The sequences and genomic features are similar to those of Scaldis River bee virus. Phylogenetic analysis based on RNA-dependent RNA polymerase, glycoprotein, and nucleoprotein sequences showed that OABV formed a subcluster within ollusviruses and was closely related to strains identified in European countries. This study extends our knowledge of wild bee parasites in Japan.

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Data availability

OABV sequence data is already available on genbank.

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Acknowledgements

This study was supported by Grant-in-Aid for Scientific Research no. 20H00425 from the Japan Society for the Promotion of Science. We are grateful to Tomoyuki Yokoi, Makihiko Ikegami, and Takaaki Suzuki for providing bee samples.

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Contributions

HT and YS designed the study. HT, YN, TY, and MO performed nucleic acid extraction, PCR, and high-throughput sequencing. HT and TM contributed to sequence data analysis. HT wrote the initial draft. YS, WH, and TM edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hitoshi Takemae.

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All authors declare that they have no conflict of interest.

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Takemae, H., Nunomura, Y., Yokota, T. et al. Novel ollusvirus detected in a solitary wild bee species (Osmia taurus) in Japan. Arch Virol 168, 183 (2023). https://doi.org/10.1007/s00705-023-05805-7

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