Abstract
The complete genome of a new virus belonging to the family Betaflexiviridae was identified in garlic and sequenced by next-generation sequencing and reverse transcription PCR. The complete RNA genome (GenBank accession number OP021693) is 8191 nucleotides in length, excluding the 3’ poly(A) tail, and contains five open reading frames (ORFs). These open reading frames encode the viral replicase, triple gene block, and coat protein, and the genome organization is typical of members of the subfamily Quinvirinae. The virus has been tentatively named "garlic yellow curl virus" (GYCV). Phylogenetic analysis suggested that it represents an independent evolutionary lineage in the subfamily, clustering with the currently unclassified garlic yellow mosaic associated virus (GYMaV) and peony betaflexivirus 1 (PeV1). Differences between the phylogenies inferred for the replicase and coat protein indicate that the new virus does not belong to any established genus of the family Betaflexiviridae. This is the first report of GYCV in China.
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Acknowledgements
We thank Prof. M. J. Adams, Minehead, UK, for correcting the English of the manuscript. This work was financially supported by the China Agriculture Research System of MOF and MARA (CARS-24-C-04), the Ningbo Major Special Projects of the Plan "Science and Technology Innovation 2025" (2021Z106), and the Key Laboratory of Biotechnology in Plant Protection, Ministry of Agriculture and Rural Affairs (D2022125) and sponsored by the K. C. Wong Magna Fund of Ningbo University.
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Peng, Z., Chen, Y., Luo, Z. et al. Complete genome sequence of a new virus from Allium sativum L in China. Arch Virol 168, 167 (2023). https://doi.org/10.1007/s00705-023-05794-7
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DOI: https://doi.org/10.1007/s00705-023-05794-7