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Complete genome sequence of a novel lytic phage of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight pathogen in rice

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Abstract

Bacteriophage L522, which infects Xanthomonas oryzae pv. oryzae, was isolated from a paddy leaf sample collected in Long An province, Vietnam. The phage shows myovirus morphology based on transmission electron microscopy. It displays a latent period and burst size of approximately 3 h and 63 new virions per infected cell (PFU/infected cell), respectively. The genome of L522 is 44,497 bp in length, with 52% GC content. Of the 63 genes identified, functions were predicted for 26. No virulence or antibiotic-resistance genes were detected. The results of a BLASTn search showed similarity to a previously reported Xanthomonas phage, with 85% average nucleotide sequence identity and 87.15% query coverage. Thus, this L522 is a representative of a new species in the genus Xipdecavirus.

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Acknowledgments

This research was funded by the Department of Science and Technology of HCMC, Vietnam, under contract number 110/2020/HĐ-QPTKHCN.

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Authors and Affiliations

  1. Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet, District 10, Ho Chi Minh City, Vietnam

    Pham D.T. My, Tu Q. Vinh, To H. Ngoc, Pham N.Q. Anh, Le T. M. Duyen, Nguyen M. Thien, Le T.T. Tien, Vo T. Phuc, Huynh N. Oanh, Le P. Nga & Hoang A. Hoang

  2. Vietnam National University Ho Chi Minh City, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam

    Pham D.T. My, Tu Q. Vinh, To H. Ngoc, Pham N.Q. Anh, Le T. M. Duyen, Nguyen M. Thien, Le T.T. Tien, Vo T. Phuc, Huynh N. Oanh, Le P. Nga & Hoang A. Hoang

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  1. Pham D.T. My
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Correspondence to Hoang A. Hoang.

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My, P.D., Vinh, T.Q., Ngoc, T.H. et al. Complete genome sequence of a novel lytic phage of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight pathogen in rice. Arch Virol 168, 157 (2023). https://doi.org/10.1007/s00705-023-05788-5

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