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Molecular characterization of a novel umbra-like virus from Thuja orientalis (arborvitae) in South Korea

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Abstract

A novel umbra-like virus was identified in arborvitae in South Korea using RNA sequencing (RNA-seq). The virus identified was tentatively named "arborvitae umbra-like virus" (AULV) and contained a 4,300-nucleotide genome organized into four non-structural open reading frames (ORFs). Cloning and Sanger sequencing were used to confirm the viral contig sequence and determine the size of the genome. Genome analysis indicated that ORF2 encodes an RNA-dependent RNA polymerase that is probably expressed through ribosomal frameshifting. ORF3 encodes a putative long-distance movement protein, while the functions of ORFs 1 and 4 are unknown. The virus lacks a coat protein gene. The genome of AULV shares 27.3%–48.4% nucleotide sequence identity with closely related umbraviruses. Phylogenetic analysis based on the complete genome sequences and amino acid sequences of the RNA-dependent RNA polymerase revealed that AULV forms a monophyletic lineage with Guiyang paspalum paspaloides tombus-like virus (GPpTV1). We suggest that AULV is a novel umbra-like virus belonging to the family Tombusviridae.

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All data generated or analysed during this study are included in this published article [and its supplementary information files].

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Acknowledgments

This work was supported by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries; Project No. AGC1762111), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. We thank Mike Herbert, PhD, from Edanz (www.edanz.com/ac) for editing a draft of this manuscript.

Funding

Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (Project No. AGC1762111).

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Authors and Affiliations

  1. Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34141, Republic of Korea

    Mesele Tilahun Belete, Jeong-A Kwon & Jae Sun Moon

  2. Plant System Engineering Research Center, Korean Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea

    Mesele Tilahun Belete, Se Eun Kim, Jeong-A Kwon, Davaajargal Igori & Jae Sun Moon

  3. Plant Biotechnology Research Division, Amhara Agricultural Research Institute, Bahir Dar, Ethiopia

    Mesele Tilahun Belete

  4. Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia

    Davaajargal Igori

  5. School of Applied Bioscience, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 98411, Republic of Korea

    Su-Heon Lee

  6. Jangchun Seed Company, 72 Sideok-ro, Yakmokmyeon, Chilgok-gun, Kyeongsangbuk-do, 39821, Republic of Korea

    Eung Kyoo Choi & Un Sun Hwang

Authors
  1. Mesele Tilahun Belete
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  2. Se Eun Kim
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  5. Eung Kyoo Choi
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Correspondence to Su-Heon Lee or Jae Sun Moon.

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Belete, M.T., Kim, S.E., Kwon, JA. et al. Molecular characterization of a novel umbra-like virus from Thuja orientalis (arborvitae) in South Korea. Arch Virol 168, 197 (2023). https://doi.org/10.1007/s00705-023-05783-w

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  • DOI: https://doi.org/10.1007/s00705-023-05783-w

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