Abstract
Two new RNA viruses were identified in Ageratum conyzoides in China using high-throughput sequencing, and their genome sequences were determined using PCR and rapid amplification of cDNA ends. The new viruses, which have positive-sense, single-stranded RNA genomes, were provisionally named “ageratum virus 1” (AgV1) and “ageratum virus 2” (AgV2). AgV1 has a genome of 3,526 nucleotides with three open reading frames (ORFs) and shares 49.9% nucleotide sequence identity with the complete genome of Ethiopian tobacco bushy top virus (genus Umbravirus, family Tombusviridae). The genome of AgV2 consists of 5,523 nucleotides and contains five ORFs that are commonly observed in members of the genus Enamovirus of the family Solemoviridae. Proteins encoded by AgV2 exhibited the highest amino acid sequence similarity (31.7–75.0% identity) to the corresponding proteins of pepper enamovirus R1 (an unclassified enamovirus) and citrus vein enation virus (genus Enamovirus). Based on their genome organization, sequence, and phylogenetic relationships, AgV1 is proposed to be a new umbra-like virus of the family Tombusviridae, and AgV2 is proposed to be a new member of the genus Enamovirus of the family Solemoviridae.
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Data availability
The genomic sequences determined in this study were submitted to the GenBank database under the accession nos. OP660856 and OP660857.
References
Okunade AL (2002) Ageratum conyzoides L. (Asteraceae). Fitoterapia 73(1):1–16
Kong C, Hu F, Xu X, Zhang M, Liang W (2005) Volatile allelochemicals in the Ageratum conyzoides intercropped citrus orchard and their effects on mites Amblyseius newsami and Panonychus citri. J Chem Ecol 31:2193–2203
Khan MS, Tiwari AK, Ji SH, Chun SC (2012) Ageratum conyzoides and its role in begomoviral epidemics; Ageratum enation virus: an emerging threat in India. Vegetos 25(2):20–28
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874
Fusaro AF, Correa RL, Nakasugi K, Jackson C, Kawchuk L et al (2012) The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation. Virology 426(2):178–187
Koonin EV, Dolja VV, Morris TJ (1993) Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol 28(5):375–430
Lu RB, Lan PX, Kang RJ, Tan GL, Chen XJ et al (2022) Genomic characterization of a new enamovirus infecting common bean. Arch Virol 167:999–1002
Acknowledgements
This work was financially supported by the earmarked fund for CARS-10-Sweetpotato and Independent Innovation Special Fund of the Henan Academy of Agricultural Sciences (2022ZC39).
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Zhao, F., Li, J., Hao, X. et al. Genomic characterization of two new viruses infecting Ageratum conyzoides in China. Arch Virol 168, 155 (2023). https://doi.org/10.1007/s00705-023-05781-y
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DOI: https://doi.org/10.1007/s00705-023-05781-y