Abstract
A bovine rotavirus (BRV) isolate from Kirsehir was isolated from feces of a neonatal calf with diarrhea, identified, and sequenced by shotgun sequencing. Its genotype constellation is G10-P[5]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The structural genes and the non-structural genes NSP1, NSP3, and NSP4 of the Kirsehir isolate were similar in sequence to those of BRVs identified in Turkey. However, VP2, NSP2, NSP4, and NSP5/6 showed similarity to those of rotaviruses from different animal hosts. These findings not only expand our current understanding of the diversity of rotaviruses but also contribute to our understanding of the evolution of rotaviruses at both the national and global levels and reinforce the significance of conducting further research on rotaviruses in Turkey.
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Acknowledgement
This work was supported by the Scientific Research Projects Coordination Unit of Kırıkkale University (project number 2021/111).
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705_2023_5778_MOESM1_ESM.xlsx
Supplementary Material 1: Amino acid sequences and amino acid substitutions in all 11 viral genomic segments of the Kirsehir isolate when compared to other selected RVA strains. The amino acid substitutions were identified using MEGA software, by selecting the “highlight variable sites” option, and the data were downloaded in Excel format and then merged into one file.
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Aksoy, E., Azkur, A.K. Genome characterization of a Turkish bovine rotavirus field isolate by shotgun metagenomics. Arch Virol 168, 159 (2023). https://doi.org/10.1007/s00705-023-05778-7
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DOI: https://doi.org/10.1007/s00705-023-05778-7