Abstract
Here, we report the detection and characterization of the genome of a novel poacevirus isolated from Zoysia matrella (Merrill) imported into the United States from Japan. The novel virus, tentatively named “zoysia mosaic virus” (ZoMV), is a single-stranded RNA virus with a genome of 9,728 nucleotides (nt) in length, encoding a large putative polyprotein of 3,119 amino acids (aa). The ZoMV genome is closely related to the triticum mosaic virus (TriMV; FJ263671) genome, with 57.18% nt and 51.74% aa sequence identity in the polyprotein region. Moreover, phylogenetic analysis showed that ZoMV is closely related to all other members of the genus Poacevirus. A survey of imported grasses showed that ZoMV was detected only in zoysiagrass. This is the first report of the complete genome sequence of a novel viral pathogen of zoysiagrass of the genus Poacevirus, for which we propose the binomial species name “Poacevirus zoisiae”.
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Data availability
The complete nucleotide sequences of ZoMV have been deposited in the NCBI GenBank database under accession number OP425115.
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Acknowledgements
The authors thank Amy Harvey-White for her help during the establishment and maintenance of the plants in the greenhouse. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer.
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Communicated by Ioannis E. Tzanetakis
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705_2023_5763_MOESM1_ESM.docx
Supplementary Table S1. Primer pairs used for amplification of genomic regions, virus detection, and 5’/3’ RACE sequencing of Zoysia mosaic virus
705_2023_5763_MOESM3_ESM.docx
Supplementary Table S2 Percentage identity of nucleotide (nt) sequences of the complete genome and amino acid (aa) sequences of the polyprotein between Zoysia mosaic virus (GenBank accession no. OP425115) and other members of the genus Poacevirus
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Adhikari, B.N., Zhou, J., Hu, X. et al. Complete genome sequence of zoysia mosaic virus, a novel member of the genus Poacevirus. Arch Virol 168, 136 (2023). https://doi.org/10.1007/s00705-023-05763-0
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DOI: https://doi.org/10.1007/s00705-023-05763-0