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Molecular characterization of a novel ourmia‑like virus from the phytopathogenic fungus Botryosphaeria dothidea

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Abstract

Here, we describe a novel ourmia-like virus, Botryosphaeria dothidea ourmia-like virus 2 (BdOLV2), derived from the phytopathogenic fungus Botryosphaeria dothidea strain ZM180192-1 infecting maize in Henan province of China. The complete genome sequence of BdOLV2 consists of a positive-sense single-stranded RNA (+ ssRNA) segment with a length of 2,532 nucleotides (nt). The sequence contains a large open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) consisting of 605 amino acids (aa) with a molecular mass of 68.59 kDa. This RdRp protein contains eight typical conserved motifs associated with ourmia-like viruses. BLASTp analysis revealed that the RdRp protein of BdOLV2 had the highest similarity (62.10%, 58.15%, and 55.75% identity, respectively) to a virus previously identified as "Botourmiaviridae sp.", Macrophomina phaseolina ourmia-like virus 2, and Macrophomina phaseolina ourmia-like virus 2-A. Phylogenetic analysis based on the RdRp aa sequence indicated that BdOLV2 is a new member of the genus Magoulivirus in the family Botourmiaviridae.

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References

  1. Zhao X, Zhang GL, Li BH, Xu XM, Dong XL, Wang CX, Li GF (2016) Seasonal dynamics of Botryosphaeria dothidea infections and symptom development on apple fruits and shoots in China. Eur J Plant Pathol 146(3):507–518. https://doi.org/10.1007/s10658-016-0935-5

    Article  CAS  Google Scholar 

  2. Phillips AJ, Rumbos IC, Alves A, Correia A (2005) Morphology and phylogeny of Botryosphaeria dothidea causing fruit rot of olives. Mycopathologia 159(3):433–439. https://doi.org/10.1007/s11046-005-0256-2

    Article  CAS  PubMed  Google Scholar 

  3. Marsberg A, Kemler M, Jami F, Nagel JH, Postma-smidt A, Naidoo S, Wingfield MJ, Crous PW, Spatafora JW, Hesse CN, Robbertse B, Slippers B (2017) Botryosphaeria dothidea: a latent pathogen of global importance to woody plant health. Mol Plant Pathol 18(4):477–488. https://doi.org/10.1111/mpp.12495

    Article  CAS  PubMed  Google Scholar 

  4. Tang W, Ding Z, Zhou ZQ, Wang YZ, Guo LY (2012) Phylogenetic and pathogenic analyses show that the causal agent of apple ring rot in China is Botryosphaeria dothidea. Plant Dis 96(4):486–496. https://doi.org/10.1094/PDIS-08-11-0635

    Article  CAS  PubMed  Google Scholar 

  5. Dong XL, Cheng ZZ, Leng WF, Li BH, Xu XM, Lian S, Wang CX (2021) Progression of symptoms caused by Botryosphaeria dothidea on apple branches. Phytopathology 111(9):1551–1559. https://doi.org/10.1094/PHYTO-12-20-0551-R

    Article  CAS  PubMed  Google Scholar 

  6. Wang L, Hou H, Zhou ZQ, Tu HT, Yuan HB (2021) Identification and detection of Botryosphaeria dothidea from Kiwifruit (Actinidia chinensis) in China. Plants (Basel) 10(2):401. https://doi.org/10.3390/plants10020401

    Article  CAS  PubMed  Google Scholar 

  7. Fan K, Wang J, Fu L, Zhang GF, Wu HB, Feng CC, Qu JL (2019) Baseline sensitivity and control efficacy of pyraclostrobin against Botryosphaeria dothidea isolates in China. Plant Dis 103(7):1458–1463. https://doi.org/10.1094/PDIS-07-18-1214-RE

    Article  CAS  PubMed  Google Scholar 

  8. Wang YZ, Zhang W, Liu BY, Luan BH, Wang PS (2010) Research on resistance and geographical distribution of Botryosphaeria dothidea from apple to tebuconazole in Shandong Province. J Fruit Sci 27(6):961–964. https://doi.org/10.13925/j.cnki.gsxb.2010.06.041

    Article  Google Scholar 

  9. Fan K, Fu L, Liu HM, Qu JL, Zhang GF, Zhang SA, Qiao K (2022) Reduced sensitivity to tebuconazole in Botryosphaeria dothidea isolates collected from major apple production areas of China. Plant Dis 106(11):2817–2822. https://doi.org/10.1094/PDIS-01-22-0053-RE

    Article  CAS  PubMed  Google Scholar 

  10. Fan HY, Ru JJ, Zhang YY, Wang Q, Li Y (2017) Fengycin produced by Bacillus subtilis 9407 plays a major role in the biocontrol of apple ring rot disease. Microbiol Res 199:89–97. https://doi.org/10.1016/j.micres.2017.03.004

    Article  CAS  PubMed  Google Scholar 

  11. Yu X, Li B, Fu YP, Jiang DH, Ghabrial SA, Li GQ, Peng YL, Xie JT, Cheng JS, Huang JB, Yi XH (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci U S A 107(18):8387–8392. https://doi.org/10.1073/pnas.0913535107

    Article  PubMed  PubMed Central  Google Scholar 

  12. Ghabrial SA, Caston JR, Jiang DH, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479–480:356–368. https://doi.org/10.1016/j.virol.2015.02.034

    Article  CAS  PubMed  Google Scholar 

  13. Wang YF, Zhao H, Cao JY, Yin XM, Guo YS, Guo LH, Wu HY, Zhang M (2022) Characterization of a novel mycovirus from the phytopathogenic fungus Botryosphaeria dothidea. Viruses 14(2):331–340. https://doi.org/10.3390/v14020331

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Mu F, Xie JT, Cheng SF, You MP, Barbetti MJ, Jia JC, Wang QQ, Cheng JS, Fu YP, Chen T, Jiang DH (2017) Virome characterization of a collection of S. sclerotiorum from Australia. Front Microbiol 8:2540. https://doi.org/10.3389/fmicb.2017.02540

    Article  PubMed  Google Scholar 

  15. Choi GH, Nuss DL (1992) Hypovirulence of chestnut blight fungus conferred by an infectious viral cDNA. Science 257(5071):800–803. https://doi.org/10.1126/science.1496400

    Article  CAS  PubMed  Google Scholar 

  16. Allen TD, Nuss DL (2004) Specific and common alterations in host gene transcript accumulation following infection of the chestnut blight fungus by mild and severe hypoviruses. J Virol 78(8):4145–4155. https://doi.org/10.1128/jvi.78.8.4145-4155.2004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Nuss DL (1991) Biological control of chestnut blight: an example of virus-mediated attenuation of fungal pathogenesis. Microbiol Rev 56(4):561–576. https://doi.org/10.1128/mr.56.4.561-576.1992

    Article  Google Scholar 

  18. Zhang HX, Xie JT, Fu YP, Cheng JS, Qu Z, Zhao ZZ, Cheng SF, Chen T, Li B, Wang QQ, Liu XQ, Tian BN, Collinge DB, Jiang DH (2020) A 2-kb mycovirus converts a pathogenic fungus into a beneficial endophyte for Brassica protection and yield enhancement. Mol Plant 13(10):1420–1433. https://doi.org/10.1016/j.molp.2020.08.016

    Article  CAS  PubMed  Google Scholar 

  19. Villamor DEV, Ho T, Al RM, Martin RR, Tzanetakis IE (2019) High throughput sequencing for plant virus detection and discovery. Phytopathology 109(5):716–725. https://doi.org/10.1094/PHYTO-07-18-0257-RVW

    Article  CAS  PubMed  Google Scholar 

  20. Chiapello M, Rodriguez-romero J, Ayllón MA, Turina M (2020) Analysis of the virome associated to grapevine downy mildew lesions reveals new mycovirus lineages. Virus Evol 6(2):veaa058. https://doi.org/10.1093/ve/veaa058

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Lian ZQ, Das S, Luo JX, Andika IB, Sun LY (2021) Complete genome sequence of a novel ourmia-like mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 166(12):3461–3465. https://doi.org/10.1007/s00705-021-05221-9

    Article  CAS  PubMed  Google Scholar 

  22. Zou Q, Gao YJ, Wang Q, Yang YK, Wang F, Hong N, Wang GP, Wang LP (2021) The full-length genome sequence of a novel mitovirus from Botryosphaeria dothidea, the causal agent of pear ring rot disease. Arch Virol 166(10):2881–2885. https://doi.org/10.1007/s00705-021-05189-6

    Article  CAS  PubMed  Google Scholar 

  23. He Y, Zou Q, Li SS, Zhu HD, Hong N, Wang GP, Wang LP (2022) Molecular characterization of a new fusarivirus infecting Botryosphaeria dothidea, the causal agent of pear ring rot disease. Arch Virol 167(9):1893–1897. https://doi.org/10.1007/s00705-022-05492-w

    Article  CAS  PubMed  Google Scholar 

  24. Li JL, Zhai LF, Zhang MX, Luo GJ, Wen YQ, Cao TT, Xia H, Zhang JY, Liu M (2022) Molecular characterization of a novel victorivirus isolated from Botryosphaeria dothidea, the causal agent of longan leaf spot disease. Arch Virol 167(11):2417–2422. https://doi.org/10.1007/s00705-022-05573-w

    Article  CAS  PubMed  Google Scholar 

  25. Yang MM, Xu WX, Zhou XQ, Yang ZK, Wang YX, Xiao F, Guo YS, Hong N, Wang GP (2021) Discovery and characterization of a novel bipartite botrexvirus from the phytopathogenic fungus Botryosphaeria dothidea. Front Microbiol 12:696125–696125. https://doi.org/10.3389/fmicb.2021.696125

    Article  PubMed  PubMed Central  Google Scholar 

  26. Ayllon MA, Turina M, Xie JT, Nerva L, Marzano SL, Donaire L, Jiang DH, Consortium IR (2020) ICTV Virus Taxonomy Profile: Botourmiaviridae. J Gen Virol 101(5):454–455. https://doi.org/10.1099/jgv.0.001409

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Yang F, Hong N, Wang GP Mycovirus dsRNA extraction kit and its application: ZL201310072994.3. 2015-06-03

  28. Zhai LF, Hong N, Zhang MX, Wang GP (2015) Complete dsRNA sequence of a novel victorivirus isolated from the pear stem wart fungus Botryosphaeria dothidea. Arch Virol 160(2):613–616. https://doi.org/10.1007/s00705-014-2285-y

    Article  CAS  PubMed  Google Scholar 

  29. Yang MM, Zhou XQ, Zhai LF, Xiao F, Hong N, Wang GP (2020) Molecular characterization of a novel mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 165(7):1667–1670. https://doi.org/10.1007/s00705-020-04629-z

    Article  CAS  PubMed  Google Scholar 

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Funding

This study was financially supported by the Open Fund of State Key Laboratory for Biology of Plant Diseases and Insect Pests (SKLOF202103) and China Agriculture Research System of MOF and MARA (CARS—27).

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Author notes

  1. Xinzheng Song and Jiayuan Cao these authors contributed equally to this study.

Authors and Affiliations

  1. College of Plant Protection, Henan Agricultural University, 450002, Zhengzhou, China

    Xinzheng Song, Jiayuan Cao, Shunpei Xie, Yanfen Wang, Xinming Yin, Yashuang Guo, Chao Xu & Meng Zhang

  2. Analytical Instrument Center, Henan Agricultural University, 450002, Zhengzhou, China

    Haiyan Wu

  3. State Key Laboratory for Biology of Plant Diseases and Insect Pests, 100193, Beijing, China

    Lihua Guo

Authors
  1. Xinzheng Song
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  2. Jiayuan Cao
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  3. Shunpei Xie
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  4. Yanfen Wang
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  5. Xinming Yin
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  6. Yashuang Guo
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  7. Chao Xu
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  9. Haiyan Wu
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  10. Meng Zhang
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Correspondence to Haiyan Wu or Meng Zhang.

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Communicated by Robert H.A. Coutts

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Song, X., Cao, J., Xie, S. et al. Molecular characterization of a novel ourmia‑like virus from the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 168, 106 (2023). https://doi.org/10.1007/s00705-023-05739-0

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