Abstract
A novel cytorhabdovirus was identified in Daphne odora in South Korea using high-throughput sequencing. The virus, tentatively named “daphne virus 1” (DV1), has a full-length genome sequence of 13,206 nucleotides with a genome organization comparable to that of unsegmented plant rhabdoviruses and contains seven antisense putative genes in the order 3′-leader-N-P′-P-P3-M-G-L-5′-trailer. The coding region of the genome is flanked by a 3′ leader and a 5′ trailer sequence, 261 and 151 nucleotides long, respectively. The DV1 genome shares 33.74%–57.44% nucleotide sequence identity with other cytorhabdoviruses. The DV1-encoded proteins share the highest amino acid sequence identity with homologues from Asclepias syriaca virus 1. Phylogenetic analysis showed that DV1 clustered with representative cytorhabdoviruses. We propose classifying DV1 in a new species within the genus Cytorhabdovirus, family Rhabdoviridae.
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Acknowledgments
This work was supported by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries; through the Crop Viruses and Pests Response Industry Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea (321104-3). We thank Steven M. Thompson from Edanz (http://www.edanz.com/ac) for editing drafts of this manuscript.
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Belete, M.T., Kim, S.E., Igori, D. et al. Complete genome sequence of daphne virus 1, a novel cytorhabdovirus infecting Daphne odora. Arch Virol 168, 141 (2023). https://doi.org/10.1007/s00705-023-05734-5
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DOI: https://doi.org/10.1007/s00705-023-05734-5