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Identification of novel totiviruses from the ascomycetous fungus Geotrichum candidum

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Abstract

Mycoviruses are widely distributed across the kingdom Fungi, including ascomycetous yeast strains of the class Saccharomycetes. Geotrichum candidum is an important fungal pathogen belonging to Saccharomycetes and has a diverse host range. Here, we report the characterization of four new classical totiviruses from two distinct Geotrichum candidum strains from Pakistan. The four identified viruses were tentatively named “Geotrichum candidum totivirus 1, 2, 3a, and 3b” (GcTV1-3b). The complete dsRNA genomes of the identified totiviruses are 4621, 4592, 4576, and 4576 bp in length, respectively. All totivirus genomes have two open reading frames, encoding a capsid protein (CP) and an RNA-dependent RNA polymerase (RdRP), respectively. The downstream RdRP domain is assumed to be expressed as a CP-RdRP fusion product via -1 frameshifting mediated by a heptameric slippery site. Sequence comparisons and phylogenetic analysis showed that each of the discovered viruses belongs to a new species of the genus Totivirus in the family Totiviridae, with GcTV1 and GcTV3 (a and b strains) clustering in one subgroup and GcTV2 in another subgroup.

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Acknowledgements

The authors are grateful to Ms. Sakae Hisano for technical assistance. HAK is thankful to the Higher Education Commission (HEC) of Pakistan for a fellowship under the International Research Support Initiative Program (IRSIP).

Funding

This investigation was partly supported by a HEC grant (no. PM-IPFP/ HRD/HEC/2012/2718 and NRPU-4109 awarded to MFB) and NUST students research funds, Grants-in-Aid for Scientific Research (S) and (A) and Grants-in-Aid for Scientific Research on Innovative Areas from the Japanese Ministry of Education, Culture, Sports, Science and Technology (KAKENHI 21H05035, 17H01463, 16H06436, 16H06429 and 16K21723 to NS and HK).

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Authors and Affiliations

  1. Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad, 44000, Pakistan

    Haris Ahmed Khan & Muhammad Faraz Bhatti

  2. Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki, 710-0046, Japan

    Hideki Kondo, Sabitree Shahi & Nobuhiro Suzuki

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  1. Haris Ahmed Khan
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  2. Hideki Kondo
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  3. Sabitree Shahi
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  4. Muhammad Faraz Bhatti
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  5. Nobuhiro Suzuki
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Contributions

Conceptualization: NS, MFB. Investigation: HAK, SS and HK. Writing—original draft: NS, HAK and MFB. Writing—reviewing and editing: NS, HAK, MFB, and HK. Funding acquisition: NS, HK, and MFB.

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Correspondence to Muhammad Faraz Bhatti or Nobuhiro Suzuki.

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Khan, H.A., Kondo, H., Shahi, S. et al. Identification of novel totiviruses from the ascomycetous fungus Geotrichum candidum. Arch Virol 167, 2833–2838 (2022). https://doi.org/10.1007/s00705-022-05611-7

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  • DOI: https://doi.org/10.1007/s00705-022-05611-7

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