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Rotavirus C infections in asymptomatic piglets in India, 2009-2013: genotyping and phylogenetic analysis of all genomic segments

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Abstract

Asymptomatic infection with rotavirus C (RVC) was observed in pigs in India, with a detection rate of 20%. Sequencing of the VP6, VP7, and NSP4 genes of RVC strains identified the genotypes I7/I10, G1, and E5, respectively. Full genome sequencing of one of these strains revealed that the genotypes of the VP4, VP1, VP2, VP3, NSP1, NSP2, NSP3, and NSP5 genes were P1, R1, C1, M3, A1, N5, T5, and H1, respectively. The detection of porcine RVC strains at two different locations in India at different time points strongly suggests that they are circulating continuously in the pig population through asymptomatic infections.

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Data availability

The datasets generated during the current study are available in the GenBank database (https://www.ncbi.nlm.nih.gov/genbank/).

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Acknowledgments

We are grateful to Dr. DT Mourya, Former Director, and Dr. SD Chitambar, Former Head, of Enteric Viruses Group ICMR-National Institute of Virology for their constant support during this period.

Funding

This work was supported by funds provided by the host institute (ICMR-National Institute of Virology, Indian Council of Medical Research, Ministry of Health and Family Welfare, Government of India).

Author information

Authors and Affiliations

  1. Enteric Viruses Group, ICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune, 411 001, India

    Madhuri S. Joshi, Shalu A. Arya, Manohar S. Shinde & Varanasi Gopalkrishna

  2. Department of Veterinary Microbiology and Animal Biotechnology, Nagpur Veterinary College, Nagpur, India

    Vijay C. Ingle

  3. Department of Animal Reproduction, Gynaecology and Obstetrics, Krantisinh Nana Patil College of Veterinary Science, Satara, India

    Hemant S. Birade

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  1. Madhuri S. Joshi
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Contributions

Conceptualization, chief investigation, analysis, manuscript writeup, review, and overall execution by MSJ. Conducting laboratory tests, analysis, compilation of data, manuscript writeup by SAA. Conducting laboratory tests, maintenance of demographic data by MSS. Systematic collection of pig faecal specimens from established pig farms by VCI and HSB. Project management, supervision, manuscript writing by VG. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Madhuri S. Joshi.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

The study was approved by the Institutional Animal Ethics committee on 3 November 2014.

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Handling Editor: Tim Skern.

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Joshi, M.S., Arya, S.A., Shinde, M.S. et al. Rotavirus C infections in asymptomatic piglets in India, 2009-2013: genotyping and phylogenetic analysis of all genomic segments. Arch Virol 167, 2665–2675 (2022). https://doi.org/10.1007/s00705-022-05607-3

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